Rat CASP3(Caspase 3) EIA Kit

• Catalog number
  E-EL-R0160-96T
• Price
  Please ask
• Size
  96Test

• Detection Type
  Colormetric
• Target Species
  Rat
• Target Synonym
  CPP32, CPP32B, SCA-1, Apoptain, Yama
• Test principle
  This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat CASP3. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CASP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CASP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CASP3. You can calculate the concentration of Rat CASP3 in the samples by comparing the OD of the samples to the standard curve.
• Sensitivity
  0.19ng/mL
• Detection Range
  0.31~20ng/mL
• Target Name
  CASP3
• UNIProt ID
  P55213
• Tested Sample Types
  Serum, Plasma, Cell supernatant
• Product Name
  Rat CASP3(Caspase 3) EIA Kit
• Type
  Sandwich
• Technical data
• Standard Curve RUL
• Description
  Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways. This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
• Properties
  MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus

• Gene target
  CASP3   Caspase   Kit  
• Gene symbol
  CASP3
• Short name
  CASP3(Caspase 3) EIA Kit
• Technique
  EIA, eia
• Host
  Rat
• Species
  Rat, Rats
• Alternative name
  Rat caspase 3, apoptosis-related cysteine peptidase(caspase 3) EIA reagent
• Alternative technique
  kits, elisas
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:1504
• Gene
  CASP3
• Long gene name
  caspase 3
• Synonyms gene name
  • caspase 3, apoptosis-related cysteine protease
  • caspase 3, apoptosis-related cysteine peptidase
• Synonyms
  • CPP32
  • CPP32B
  • Yama
  • apopain
• GenBank acession
  • BC016926
• Locus
  4q35.1
• Discovery year
  1996-07-22
• Entrez gene record
  836
• Pubmed identfication
  • 8780721
• RefSeq identity
  • NM_004346
• Classification
  • Caspases
  • Small nucleolar RNA protein coding host genes
• VEGA ID
  OTTHUMG00000133681

MeSH Data

• Name
  Models, Chemical
• Concept
  Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
• Tree numbers
  • E05.599.495

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