Rabbit Anti Human RB1 Antibody-Non-conjugated
-
Catalog numberCSB-PA019386ESR2HU_50ul
-
PricePlease ask
-
Size50ul
-
-
Long nameRabbit Antibody against Human RB1, Non-conjugated
-
Synonym nameRB1 Human Antibody
-
Species reactivityHuman
-
Tested applications recommended dilutionsELISA,IHC;Recommended dilution:IHC:1:20-1:200
-
ConjugationNon-conjugated
-
Antibody isotypeIgG
-
UniProt IDP06400
-
FormatLiquid, diluted in buffer
-
Buffer compositionPBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Purity>95%
-
Purification methodAntigen Affinity Purified Antibodies
-
Antibody categoryPolyclonal Antibodies
-
Immunogen typeRecombinant human Retinoblastoma-associated protein (649-928AA)
-
Immunogen speciesHuman
-
Aliasp105-Rb,pRb,Rb,pp110,RB1
-
BackgroundKey regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
-
ClonalityPolyclonal antibody
-
Shipping and storageRB1 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
-
PropertiesIf you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
AboutRabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin nameOryctolagus cuniculus
-
French translationanticorps
-
Gene target
-
Gene symbolRB1-DT, RB1
-
Short nameAnti-RB1
-
Techniqueanti human, Antibody, Rabbit, anti, antibody to, anti human antibodies, antibodies against human proteins, antibodies for, antibody Conjugates
-
HostRabbit, Rabbits
-
SpeciesHuman, Humans
-
Alternative nameRB1 Antibody
-
Alternative techniqueantibodies, rabbit-anti
-
Alternative to gene targetretinoblastoma 1, OSRC and p105-Rb and pp110 and PPP1R130 and pRb and RB, RB1 and IDBG-32092 and ENSG00000139687 and 5925, phosphoprotein binding, nuclei, Rb1 and IDBG-179829 and ENSMUSG00000022105 and 19645, BT.68871 and IDBG-629082 and ENSBTAG00000006640 and 534712
-
Gene info
-
Identity
-
Gene
-
Long gene nameRB1 divergent transcript
-
Synonyms gene
-
Synonyms gene name
- long intergenic non-protein coding RNA 441
-
Synonyms
-
Locus
-
Discovery year2011-08-18
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Divergent transcripts
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameRB transcriptional corepressor 1
-
Synonyms gene
-
Synonyms gene name
- osteosarcoma
- retinoblastoma 1
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1986-01-01
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Receptor ligands
-
VEGA ID
-
Locus Specific Databases
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
