-
Long name
Rabbit Antibody against Human DDX42, HRP
-
Synonym name
DDX42 Human Antibody
-
Species reactivity
Human
-
Tested applications recommended dilutions
ELISA
-
Conjugation
HRP
-
Antibody isotype
IgG
-
UniProt ID
Q86XP3
-
Format
Liquid, diluted in buffer
-
Buffer composition
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
-
Purity
>95%
-
Purification method
Protein G purified
-
Antibody category
Polyclonal Antibodies
-
Immunogen type
Recombinant human ATP-dependent RNA helicase DDX42 protein (519-819AA)
-
Immunogen species
Human
-
Alias
ATP-dependent RNA helicase DDX42 (EC:3.6.4.13),DDX42,DEAD box protein 42,RNA helicase-like protein,RHELP,RNA helicase-related protein,RNAHP,SF3b DEAD box protein,Splicing factor 3B-associated 125 kDa protein,SF3b125
-
Background
ATP-dependent RNA helicase. Binds to partially double-stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. Unwinding is promoted in the presence of single-strand binding proteins. Mediates also RNA duplex formation thereby displacing the single-strand RNA binding protein. ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands. Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2. Relocalizes TP53BP2 to the cytoplasm.
-
Clonality
Polyclonal antibody
-
Shipping and storage
DDX42 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
-
Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Additional conjugation
HRP Conjugated
-
About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
-
French translation
anticorps
-
Gene target
-
Gene symbol
DDX42
-
Short name
Anti-DDX42
-
Technique
anti human, Antibody, Rabbit, anti, antibody to, anti human antibodies, antibodies against human proteins, antibodies for
-
Host
Rabbit, Rabbits
-
Label
HRP
-
Species
Human, Humans
-
Alternative name
DDX42 Antibody,HRP conjugated
-
Alternative technique
antibodies, rabbit-anti
-
Alternative to gene target
DEAD (Asp-Glu-Ala-Asp) box polypeptide 42, DDX42P and RHELP and RNAHP and SF3b125 and SF3B8, DDX42 and IDBG-63647 and ENSG00000198231 and 11325, poly(A) RNA binding, nuclei, Ddx42 and IDBG-213407 and ENSMUSG00000020705 and 72047, DDX42 and IDBG-641673 and ENSBTAG00000021058 and 100140430
-