Phosphatidylglycerol/Cardiolipin Assay test kit

  • Catalog number
    MET-5024
  • Price
    Please ask
  • Size
    100 assays
  • We send this reagant on
    Dry Ice
  • The temperatureerature for storage is
    Store the entire test kit at -80ºC. Avoid multiple freeze/thaws by aliquoting. The Fluorimetry Probe is light sensitive and should be maintained in amber tubes.
  • Technical file
    more kit-Fluorimetry.pdf
  • SDS or safty data sheet
    details (MET-5024) GHS.pdf
  • Type of reagent
    Metabolism Research
  • Related techniques
    Metabolism Research, Lipoprotein Metabolism, Phospholipid Assays
  • Use of the reagent
    Our Phosphatidylglycerol/Cardiolipin Assay test kit measures both phosphatidylglycerol and cardiolipin in cell lysate samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidylglycerol and cardiolipin to glycerol, which is then phosphorlyated by glycerol kinase to yield glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the test kit’s Fluorimetry Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidylglycerol and cardiolipin levels in unknown sampled are determined based on the provided cardiolipin standard curve.
  • Cellular use
    Detects phosphatidylglycerol and cardiolipin in cell lysate samples
  • Protocol information
    Detection sensitivity of approximately 1 uM
  • Reagent properties
    Cardiolipin standard curve included
  • Conjugation and form
    host specific
  • Species compatible to
    human cells and cell lines and other cells
  • Use of this test kit or product
    Molecular biology
  • Antigen
    the sequence of the protein
  • Buffer
    The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,7
  • Molecular weight
    kiloDalton
  • This reagent is purified
    0.98
  • Test
    Cellular biology labs supplies other types of Assays as 1.
  • Gene target
  • Short name
    Phosphatidylglycerol/Cardiolipin Assay test kit
  • Technique
    Assay, assays
  • Host
    Assay
  • Alternative name
    Phosphatidylglycerol/Cardiolipin test test reagent
  • Alternative technique
    arrays, tests, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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