Phosphatidylglycerol Assay Kit (Fluorometric)

• Catalog number
  K488-100
• Price
  Please ask
• Size
  100 assays

• Description
  The first kit in the market that detects Phosphatidylglycerol levels in biological samples
• Summary
  Phosphatidylglycerol Assay Kit (Fluorometric): Fluorometric Assay for measuring PG levels in biological samples such as tissue lysate. Detection limit: 0.02 nmol per sample. 100 assays.
• Detection Method
  Fluorometric (535/587 nm)
• Species Reactivity
  All
• Applications
  Measurement of PG content of various tissue/cell extracts, Determination of PG concentration in biological fluids
• Sample Type
  Cell lysate
• Features Benefits
  •Can be completed in less than one hour•Specific•validated with biological samples•Simple•easy protocols
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Phospholipids are physiologically critical amphiphilic molecules that are involved in regulation of numerous cellular processes. Phosphatidylglycerol (PG) is a glycerophospholipid consisting of a glycerol backbone with two acyl chains and a phosphatidyl group that carries another glycerol moiety. Lung surfactant is an important lipid-protein mixture that aids in expansion and prevents total collapse of the lung during respiration. PG is a prominent phospholipid in lung surfactant, and its presence can be monitored as a measure of lung development in the maturing fetus. In addition, most bacteria utilize PG in their membrane, and plants utilize this component as the major phospholipid in the photosynthetic membranes of leaf tissue. In HPLC analysis of phospholipids, phosphatidic acid can interfere with and complicate determination of PG content. BioVision’s Phosphatidylglycerol Assay Kit utilizes specific enzymes to generate an intermediate that reacts with a probe, yielding a signal that can be quantified fluorometrically, and is proportional to the amount of PG present in the sample, unaffected by the presence of phosphatidic acid, cardiolipin, or other lipids. When used as described, the assay is highly selective against other phospholipids (see figure below) and capable of detecting as little as 20 pmoles of phosphatidylglycerol.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Phosphatidylglycerol   Kit   Fluorometric  
• Short name
  Phosphatidylglycerol Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Phosphatidylglycerol test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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