OxiSelect Intracellular ROS Assay Kit (Green Fluorescence)
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Catalog numberSTA-342-5
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PricePlease ask
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Size5 x 96 reactions
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Type of the productSet of reagents
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Product descriptionThe OxiSelect ROS Assay Set of reagents is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.
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Shipping conditionsThe product OxiSelect Intracellular ROS Assay Kit (Green Fluorescence)can be shipped at ambient temperature without any risk for the quality and activity of the reagents.
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Storage conditionsAfter the delivery of this product, our specialists from Gentaur/Genprice recommend you to prepare aliquots to minimize the risk of repeated cycles of freezing and thawing. The standard suspension should be kept at minus twenty degrees Celsius but the rest of the reagents can be stored at four degrees Celsius.
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TipsThis product is manufactured for research purposes only and it is not intended to be used in clinical diagnostic procedures.
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TestCellular biology labs supplies other types of Assays as 1. Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
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Gene target
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Gene symbolROS1, GUCY2F, GUCY2D
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Short nameOxiSelect Intracellular ROS Assay Kit ( )
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Techniqueintracellular, Assay, assays
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HostAssay
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Labelgreen
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Alternative nameOxiSelect Intracellular ROS test reagent (Green Fluorescence)
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Alternative techniquearrays, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene nameROS proto-oncogene 1, receptor tyrosine kinase
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Synonyms gene name
- v-ros avian UR2 sarcoma virus oncogene homolog 1
- v-ros UR2 sarcoma virus oncogene homolog 1 (avian)
- c-ros oncogene 1 , receptor tyrosine kinase
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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Classification
- Receptor tyrosine kinases
- Fibronectin type III domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameguanylate cyclase 2F, retinal
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-12-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Guanylate cyclase receptors
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameguanylate cyclase 2D, retinal
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Synonyms gene
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Synonyms gene name
- cone rod dystrophy 6
- guanylate cyclase 2D, membrane (retina-specific)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-11-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Guanylate cyclase receptors
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VEGA ID
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Locus Specific Databases