OxiSelect Intracellular Nitric Oxide (NO) Assay Kit (Fluorometric)
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Catalog numberSTA-800-5
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Price:
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Size5 x 96 reactions
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Type of the productSet of reagents
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Product descriptionThe OxiSelect Intracellular Nitric Oxide Assay Set of reagents is a cell-based assay for rapid detection of intracellular NO or NOS activity in cultured cells. In brief, the cell-permeant NO probe passively diffuses into cells and is deacetylated by cellular esterases to a non-fluorescent intermediate. When intracellular nitric oxide encounters this intermediate, it rapidly oxidizes to a highly fluorescent, triazolo-fluorescein analog. The fluorescence intensity is proportional to the NO levels within the cell cytosol (detection limit of ~3 nM). The Set of reagents’s provided Nitric Oxide Fluorometric Probe is NO specific, has excellent photostablity and pH stability, and is detected with standard fluorescein filters. The probe is suitable for flow cytometry, fluorescent microscopy, and fluorescent microplate detection. Each Set of reagents provides sufficient reagents to perform up to 96 reactions, including blanks, standards, and unknown samples.
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Shipping conditionsThe product OxiSelect Intracellular Nitric Oxide (NO) Assay Kit (Fluorometric) must be shipped on blue ice packages to retain the activity and stability of the reagents.
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Storage conditionsAfter the delivery of this product, our specialists from Gentaur/Genprice recommend you to prepare aliquots to minimize the risk of repeated cycles of freezing and thawing. The standard suspension should be kept at minus twenty degrees Celsius but the rest of the reagents can be stored at four degrees Celsius.
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TipsThis product is manufactured for research purposes only and it is not intended to be used in clinical diagnostic procedures.
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TestCellular biology labs supplies other types of Assays as 1.
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Gene target
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Short nameOxiSelect Intracellular Nitric Oxide (NO) Assay Kit (Fluorometric)
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Techniqueintracellular, Assay, assays
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HostAssay
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Alternative nameOxiSelect Intracellular Nitric Oxide (NO) test reagent (fluorescence)
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Alternative techniquearrays, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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