OxiSelect Intracellular Nitric Oxide (NO) Assay Kit (Fluorometric)
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Catalog number
STA-800-5
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Price
Please ask
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Size
5 x 96 assays
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Shipping Temp
Blue Ice
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Storage Temp
Store the entire kit at -20ºC
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Category Product Type
Kit
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URL link to SDS
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UNSPSC
41116100
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Product Line
Oxidative Stress/Damage
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Short Description
The OxiSelect™ Intracellular Nitric Oxide Assay Kit is a cell-based assay for rapid detection of intracellular NO or NOS activity in cultured cells. In brief, the cell-permeant NO probe passively diffuses into cells and is deacetylated by cellular esterases to a non-fluorescent intermediate. When intracellular nitric oxide encounters this intermediate, it rapidly oxidizes to a highly fluorescent, triazolo-fluorescein analog. The fluorescence intensity is proportional to the NO levels within the cell cytosol (detection limit of ~3 nM). The kit’s provided Nitric Oxide Fluorometric Probe is NO specific, has excellent photostablity and pH stability, and is detected with standard fluorescein filters. The probe is suitable for flow cytometry, fluorescent microscopy, and fluorescent microplate detection. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, standards, and unknown samples.
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Product feature 1
Direct, rapid quantitation of intracellular nitric oxide in cultured cells
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Product feature 2
Detects nitric oxide as low as 3 nM
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Product feature 3
Fluorometric probe is nitric oxide-specific and has excellent photostability and pH stability
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Test
Cell Biolabs supplies other types of Assays as 1.
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Gene target
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Short name
OxiSelect Intracellular Nitric Oxide (NO) Assay Kit (Fluorometric)
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Technique
intracellular, Assay, assays
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Host
Assay
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Alternative name
OxiSelect Intracellular Nitric Oxide (NO) test reagent (fluorescence)
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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