Nitric Oxide Fluorometric Assay Kit
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Catalog number
K252-200
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Price
Please ask
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Size
200 assays
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Description
Detects total nitrate/nitrite
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Summary
• Detection method- Fluorescence (Ex/Em 380/461 nm) • Species reactivity- Mammalian • Applications- Measurement of total nitrate/nitrite concentration
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Sample Type
Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
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Features Benefits
• Simple procedure; takes only 2-3 hours • Fast and convenient • The fluorometric kit provides a simple two-step process for quantitative measurement of total nitrate/nitrite productions. The assay can be easily done in a 96-well plate.
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. Since NO is rapidly converted to nitrite (NO²ˉ) and nitrate (NOᶟˉ), the total concentration of nitrite and nitrate is used as a quantitative measure of NO production. BioVision’s Nitric Oxide Fluorometric Assay Kit provides an accurate and convenient measurement of total nitrate/nitrite concentration in a simple two-step process. In the first step nitrate is converted to nitrite by nitrate reductase. In the second step, nitrite reacts with the fluorescent probe DAN (2, 3 diaminonaphthalene). NaOH enhances the fluorescent yield. The fluorescent intensity is proportional to the total nitric oxide production. The kit has been tested with culture media, plasma, and tissue homogenates.
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Short name
Nitric Oxide Fluorometric Assay Kit
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Technique
Assay, assays
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Host
Assay
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Alternative name
Nitric Oxide fluorescence test reagent
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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