β-Xylosidase Activity Assay Kit (Fluorometric)
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Catalog number
K981-100
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Price
Please ask
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Size
100 assays
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Summary
• Detection method- Fluorescence (Ex/Em 330/450 nm) • Applications- Measurement of β-Xylosidase activity in various biological samples and purified/crude enzyme preparations
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Detection Method
Fluorescence (Ex/Em 330/450 nm)
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Species Reactivity
Bacterial
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Applications
Measurement of β-Xylosidase activity in various biological samples and purified/crude enzyme preparations
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Sample Type
• Environmental Samples • Purified enzyme
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Features Benefits
• Simple, rapid & convenient assay to measure β-Xylosidase activity • Includes Positive Control (β-Xylosidase)
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
β-Xylosidase (EC 3.2.1.37) is a hydrolytic enzyme responsible for the breakdown of hemicellulose, a primary component of the plant cell wall. Specifically, β-Xylosidases remove the terminal β-xylose residue from the non-reducing terminus of a polysaccharide chain. As xylan is the principal variety of hemicellulose present in the plant cell wall, the xylosidase activity is the final step in the enzymatic generation of the five carbon reducing sugar, xylose. Individual reducing sugars can be converted to ethanol, which serves as a building block for biofuels. Xylose can also be converted into other value-added chemicals such as Xylitol and Xylonic Acid. BioVision’s β-Xylosidase Activity Assay Kit provides a quick, reliable, and sensitive fluorometric method for the determination of -Xylosidase activity. A fluorogenic substrate is incubated with the sample, and in the presence of β-Xylosidase activity, the substrate is cleaved, releasing a fluorophore (Ex Em 330/450 nm). The increase in fluorescence is a direct measure of the β-Xylosidases activity in the sample. When used according to protocol, activities as low as one µUnit (1 pmol/min) in environmental microbial samples, such as compost, can be detected.
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Short name
β-Xylosidase Activity Assay Kit (Fluorometric)
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Technique
Assay, assays
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Host
Assay
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Alternative name
β-Xylosidase Activity test reagent (fluorescence)
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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