Total Phosphodiesterase Activity Assay Kit (Fluorometric)

• Catalog number
  K927-100
• Price
  Please ask
• Size
  100 assays

• Description
  Highly Sensitive, minimal sample preparation required
• Summary
  • Detection method - Fluorescence (Ex/Em 370/450 nm) • Species reactivity - Mammalian • Applications - • This assay has a detection sensitivity as low as 0.25 µU.
• Detection Method
  Fluorescence (Ex/Em 370/450 nm)
• Species Reactivity
  Mammalian
• Applications
  Detection and quantificiation of total phosphodiesterase activity in biological samples
• Sample Type
  Mammalian
• Features Benefits
  • Simple, highly sensitive, high-throughput compatible • Detection and quantificiation of total phosphodiesterase activity in biological samples • This assay has a detection sensitivity as low as 0.25 µU.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Phosphodiesterase (PDE) is a family of enzymes that cleave a phosphodiester bond. These include cyclic nucleotide phosphodiesterases, phospholipase C and D, RNases, DNases and some restriction endonucleases. Phosphodiesterases are important enzymes used for a variety of biological functions including metabolism of second messenger molecules like cAMP / cGMP (therefore, regulation of their intracellular levels). PDE activity is responsible for degradation of mRNA after it had been translated to protein and of DNA during apoptosis. Some PDE isoforms such as PDE 5 and PDE 11 are therapeutic targets because of their role in the cardiovascular system and tumor formation respectively. BioVision’s PDE assay kit is a fluorometric plate-based assay for kinetic measurement of total phosphodiesterase activity. It is based on the cleavage of a coumarin based synthetic substrate by phosphodiesterases to generate a product, which fluoresces at Ex/Em= 370/450 nm. It does not rely on the quantification of phosphate released by phosphodiesterase action, like other traditional methods currently in use. Therefore, sample background is negligible, and does not interfere with the assay. The substrate is specific for phosphodiesterases and is not cleaved by enzymes that act on carboxyl esters or by phosphatases. This assay can detect as low as 0.25 µU of total PDE.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Phosphodiesterase   Activity   Kit   Fluorometric  
• Short name
  Phosphodiesterase Activity Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Total Phosphodiesterase Activity test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  total

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