Sirtuin Activity Assay Kit (Fluorometric)

• Catalog number
  K324-100
• Price
  Please ask
• Size
  100 assays

• Description
  Validated using many sample types
• Summary
  • Detection method- Fluorescence (Ex/Em = 400/505 nm) • Application- Detection of Sirtuin Activity in variety of samples
• Detection Method
  Fluorescence (Ex/Em = 400/505 nm)
• Applications
  Detection of Sirtuin Activity in variety of samples
• Sample Type
  • Purified recombinant protein • Cell and tissue lysate • Nuclear Extract • Mitochondria • Immunoprecipitated samples
• Features Benefits
  • Rapid, and convenient • The assay can detect Sirtuin activity as low as 0.06 µU of recombinant human SIRT6.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Sirtuins are a class of proteins that possess either histone deacetylase or mono-ribosyltransferase activity. Sirtuins are localized in the cytoplasm, nucleus, nucleolus as well as mitochondria. They are associated with aging, cellular protection, sugar metabolism and cell cycle regulation. Unlike other known protein deacetylases, which simply hydrolyze acetyl-lysine residues, the sirtuin-mediated deacetylation reaction hydrolyzes acetyl-lysine and NAD. This hydrolysis yields the deacetylated substrate, O-acetyl-ADP-ribose and nicotinamide, itself an inhibitor of sirtuin activity. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. In BioVision’s Sirtuin Activity Assay Kit, the acetylated p53-AFC substrate is deacetylated by Sirtuins in the presence of NAD+ to generate the deacetylated p53-AFC substrate, nicotinamide and O-Acetyl-ADP Ribose. Cleavage of the deacetylated p53-AFC substrate by the Developer releases the fluorescent group, which is detected fluorometrically at Ex/Em = 400/505 nm. HDAC’s also deacetylate the acetylated p53-AFC substrate. Trichostatin A is added to the reaction to specifically inhibit HDAC’s in samples. This kit provides a rapid, simple, sensitive, and reliable test to measure Sirtuin Activity in a variety of samples. The limit of quantification of the assay is 0.06 µU of recombinant human SIRT6.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Sirtuin   Activity   Kit   Fluorometric  
• Short name
  Sirtuin Activity Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Sirtuin Activity test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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