RasGAP Antibody
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Catalog number3311-30T
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PricePlease ask
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Size30 µg
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Antibody TargetRasGAP
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Target Alternative NameRASGAP, p120GAP , RASA1, RASA, CMAVM, CM-AVM , PKWS , p120GAP, DKFZp434N071
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat, bovine
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Immunogen SequenceSynthetic peptide surrounding amino acid 1025 of human RasGAP
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AccessionP20936
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Gene Id5921
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AppearanceColorless liquid
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FormLiquid
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Concentration0.2 mg/ml
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Formulation30 µg (0.2 mg/ml) protein A purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blot, Immunoprecipitation, Immunohistochemistry
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Application And UsagesWestern blotting (0.5-4 µg/ml) and in immunoprecipitation (10-20 µg/ml) and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate can be used as a positive control. The antibody recognizes ~120 kDa Ras GAP of human, mouse, rat, and bovine origins.
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DescriptionThe mammalian proto-oncogene Ras encodes 21 kDa proteins (p21Ras) which exists in either a physiologically quiescent GDP-binding state or a GTPbinding signal-emitting state. Interaction of p21Ras with GTPase activating protein (GAP) can increase hydrolysis of p21Ras-bound GTP by as much as 1000-fold. The product of the neurofibromatosis type 1 gene (NF1) has also been shown to exhibit p21Ras GAP activity.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolIQGAP1
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Short nameRasGAP Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameRasGAP (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameIQ motif containing GTPase activating protein 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-07-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- IQ motif containing GTPase activating protein family
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data