Proteasome Activity Fluorometric Assay Kit

• Catalog number
  K245-100
• Price
  Please ask
• Size
  100 assays

• Description
  Specifically detects proteasome activity
• Summary
  • Detection method- Fluorescence (Ex/Em 350/440nm) • Species reactivity- Mammalian • Applications- Specifically measures proteasome activity from other protease activity present in samples.
• Sample Type
  Whole cell lysates, nuclear and cytoplasmic lysates
• Features Benefits
  • Simple one-step procedure; takes only 30-60 minutes • Accurate and easy to use
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Proteasomes are very large (20S, 26S) protein assemblies found in both the nucleus and cytoplasm of all eucaryotes (and in some procaryotes) which are responsible for the degradation and recycling of proteins which have been previously tagged with ubiquitin. Such tagged proteins are degraded into peptides approximately 7-8 amino acids long which are subsequently further degraded. The 20S assembly is the functional protease structure with chymotrypsin-like, trypsin-like and caspase-like protease activities. BioVision’s Proteasome Activity Assay takes advantage of the chymotrypsin-like activity, utilizing an AMC-tagged peptide substrate which releases free, highly fluorescent AMC in the presence of proteolytic activity. The kit also includes a positive control (Jurkat Cell lysate with significant proteasome activity) and a specific proteasome inhibitor mg-132 which suppresses all proteolytic activity due to proteasomes, which make the assay specifically measures proteasome activity from other protease activity which may be present in samples.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Proteasome   Activity   Fluorometric   Kit  
• Short name
  Proteasome Activity Fluorometric Assay Kit
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  protein degradation aggregate Activity fluorescence test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Product images

Similar products