Prostate Specific Antigen (PSA) Activity Assay Kit (Fluorometric)
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Catalog number
K962-100
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Price
Please ask
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Size
100 assays
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Description
Highly Sensitive, minimum sample preparation required
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Summary
• Detection method - Fluorescence (Ex/Em = 360/460 nm) • Applications- Detection of ACE1 activity in seminal fluid and purified enzyme
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Detection Method
Fluorometric (Ex/Em = 360/460 nm)
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Species Reactivity
Mammalian
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Applications
Detection of PSA activity in seminal fluid and purified enzyme
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Sample Type
• Purified Enzyme • Seminal Fluid
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Features Benefits
• Simple one-step reaction • Takes only 1-2 hrs • Non-radiometric fluorescent detection • HTP adaptable
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Prostate Specific Antigen (PSA, EC 3.4.21.77), or Kallikrein-related peptidase 3, is a serine protease. It proteolyses the Semenogelin I and Semenogelin II of seminal fluid and thus liquefies it. PSA also shows antiangiogenic activity. The significance of the level of enzymatically active PSA is emerging in diagnosis and prognosis of prostate cancer. Growing number of evidence suggests that PSA plays an important role in prostate tumor growth, invasion and metastasis. BioVision’s PSA Activity Assay Kit enables the research in this field. This kit utilizes the ability of an active PSA to cleave a synthetic AMC based peptide substrate to release a free fluorophore. The released AMC can be easily quantified using a fluorescent microplate reader. This assay kit is simple and can be used in a high-throughput format. This assay can detect as low as 1 ng of purified active PSA.
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Additional description
Antigens are peptides or recombinant or native dependent on the production method.
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Gene symbol
KLK3, PSAT1, NPEPPS
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Short name
Prostate Specific Antigen (PSA) Activity Assay Kit (Fluorometric)
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Technique
antigen, Assay, antigenes, assays
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Host
Assay
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Alternative name
Prostate Specific protein (PSA) Activity test reagent (fluorescence)
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Alternative technique
antigenes, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
prostate
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Gene info
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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