PPDK (Pyruvate, phosphate dikinase) Activity Assay Kit (Fluorometric/Colorimetric)

• Catalog number
  K456-100
• Price
  Please ask
• Size
  100 assays

• Summary
  • Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity- Mammalian • Applications- The kit detects 6 mU PPDK activity.
• Detection Method
  Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Species Reactivity
  Bacterial/Plants
• Applications
  • Measurement of PPDK activity in tissue/cell lysates and protein preparations • Screening for specific inhibitors of PPDK activity that may affect microorganisms/plant biochemical pathways • Study of pyrophosphate-mediated glycolysis in bacterial and protozoan cell lysates • Detection of activity and light activation kinetics of PPDK from crude leaf extracts and recombinant protein • Study of photosynthesis in plants
• Sample Type
  • Tissue culture extracts and lysates; protein preps
• Features Benefits
  • Simple procedure; takes ~ less than 60 minutes • Fast and convenient
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Pyruvate, phosphate dikinase, PPDK, (E.C. 2.7.9.1) is found in microorganisms and plants where it catalyzes reversible interconversion of AMP, PPi and phosphoenolpyruvate (PEP) to ATP, Pi and pyruvate monophosphate. PPDK plays an important role in plant photosynthesis and glycolytic pathway of endobiotic protozoa and bacteria where pyruvate, phosphate dikinase (PK) is absent; it functions in the process of ATP biosynthesis. In plants, PPDK is a pivotal regulator of the C4 photosynthetic cycle and the key limiting factor for maintaining photosynthesis at low temperature. It catalyzes the regeneration of PEP as the primary acceptor of atmospheric CO2 from pyruvate, ATP and phosphate. In addition, PPDK is also present in C3 leaves where it confers a similar light/dark regulation in chloroplasts, and in wheat leaves in an amount comparable to that found in seeds. Since PPDK is not found in vertebrates, finding its inhibitors may be useful for treatment of infections caused by anaerobic protists that depend on pyrophosphate-dependent glycolysis. Specific inhibition of PPDK is expected to inhibit Giardia and other anaerobic protists such as Entamoeba histolytica and Trichomonas vaginalis without harming their mammalian hosts. BioVision’s Pyruvate, phosphate dikinase, (PPDK) Assay Kit provides a simple and rapid test for measuring specific activity of PPDK in bacterial, protozoan and plant lysates as well as protein preps. We utilize the ability of PPDK to interconvert the substrate into intermediate product detected by proprietary enzyme mix and probe. Generated stable product can be quantified by either colorimetric or fluorometric readout. The assay is simple to perform, high-throughput adaptable and a fluorometric reaction can detect as low as of 0.006 U of PPDK activity in a single well. We provide sufficient reagents for 100 fluorometric or colorimetric assays.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  PPDK   Pyruvate   phosphate   dikinase   Activity   Kit   Fluorometric  
• Short name
  PPDK (Pyruvate, phosphate dikinase) Activity Assay Kit (Fluorometric/Colorimetric)
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  PPDK (Pyruvate, phosphate dikinase) Activity test reagent (fluorescence/colorimetry)
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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