Phenylalanine Ammonia-Lyase Activity Assay Kit (Fluorometric)

• Catalog number
  K2055-100
• Price
  Please ask
• Size
  100 assays

• Description
  To measure the activity of Phenylalanine Ammonia-Lyase in samples
• Summary
  BioVision's Phenylalanine Ammonia-Lyase Activity Assay Kit measures the activity of PAL via a two-step plate-based assay. The products of the PAL enzymatic reaction, generated in the first step, react with the developers resulting in the formation of a fluorescent compound, which is detected at Ex/Em = 410/470 nm. The kit can detect as low as 3 µUnits (3 pmol substrate catalysis per minute) of PAL under assay conditions.
• Detection Method
  Fluorescent (Ex/Em = 410/470 nm)
• Species Reactivity
  Mammalian
• Applications
  Measurement of Phenylalanine ammonia-lyase activity in samples
• Sample Type
  Purified protein or recombinant enzyme (must be free of primary amines including ammonium sulfate),Serum
• Features Benefits
  The assay is simple, sensitive, and can detect as low as 3 µU (3 pmol substrate catalysis/minute) of PAL under assay conditions.,A facile, rapid way to measure Phenylalanine ammonia-lyase activity in samples.
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Phenylalanine Ammonia-Lyase (PAL, EC 4.3.1.24) catalyzes the reversible non-oxidative deamination of L-phenylalanine to trans-cinnamic acid and ammonia without any external cofactors. PAL is expressed in plants, yeast and some fungi and is absent in bacteria and animals. Patients suffering from the genetic disorder, phenylketonuria is unable to metabolize phenylalanine that leads to irreversible cognitive impairment. Historically, phenylketonuria has been treated with a phenylalanine-restricted diet taken for the entirety of the patient’s life. Recently, a drug containing PEGylated PAL has been FDA-approved to treat phenylketonuria, limiting the need of a restricted diet. BioVision's Phenylalanine Ammonia-Lyase Activity Assay Kit measures the activity of PAL via a two-step plate-based assay. The products of the PAL enzymatic reaction, generated in the first step, react with the developers resulting in the formation of a fluorescent compound, which is detected at Ex/Em = 410/470 nm. The kit can detect as low as 3 µUnits (3 pmol substrate catalysis per minute) of PAL under assay conditions.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Phenylalanine   Ammonia-Lyase   Activity   Kit   Fluorometric  
• Short name
  Phenylalanine Ammonia-Lyase Activity Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Phenylalanine Ammonia-Lyase Activity test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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