Peroxidase Activity Colorimetric/Fluorometric Assay Kit

• Catalog number
  K772-100
• Price
  Please ask
• Size
  100 assays

• Description
  Highly Sensitive Assay, HTS
• Summary
  • Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em=535/587) • Species reactivity- Mammalian • Application- The kit has a detection limit of 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.
• Sample Type
  Cell and tissue culture supernatant, urine, serum and plasma, as well as many other biological fluids
• Features Benefits
  • Simple procedure • Fast and convenient • The assay is simple, direct, highly sensitive and high-throughput adaptable
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form: ROOR' + electron donor (2 e-) + 2H+ → ROH + R'OH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues. BioVision’s Peroxidase Assay Kit provides a convenient colorimetric and fluorometric means to measure the peroxidase activity in biological samples. In the presence of Peroxidase, the OxiRed Probe reacts with H2O2 in a 1:1 stoichiometry to produce the red-fluorescent oxidation product, resorufin. The resorufin is quantified by colorimetric (lmax = 570nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, direct, highly sensitive and high throughput-ready. The detection limit is 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.
• Conjugate
  Peroxidases with EC number 1.11.1.x are catalyzing the reaction of ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH

• Gene target
  Activity   Fluorometric   Kit  
• Short name
  Activity Colorimetric/Fluorometric Assay Kit
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Label
  peroxidase
• Alternative name
  Peroxidase Activity colorimetry/fluorescence test reagent
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Product images

Similar products