Pepsin/Pepsinogen Activity Assay Kit (Fluorometric)

• Catalog number
  K446-100
• Price
  Please ask
• Size
  100 assays

• Description
  Highly sensitive and specific
• Summary
  • Detection method- Fluorescence (Ex/Em: 328/418 nm) • Species reactivity- Mammalian • Application- The kit detects as low as 500 µU pepsin in various samples.
• Detection Method
  Fluorescence (Ex/Em 328/418 nm)
• Species Reactivity
  Mammalian
• Applications
  Estimation of pepsin activity in various biological samples
• Sample Type
  • Mammalian gastric tissues (stomach, duodenum, etc.) • Human biological fluids (serum/plasma, gastric juice, vomit)
• Features Benefits
  • Simple procedure; takes ~ 1-2 hours based on pepsin levels • Fast and convenient
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Pepsin (EC 3.4.23.1) is a peptidase A1 family aspartic protease that serves as a primary digestive enzyme in the mammalian stomach. Pepsin is synthesized in the chief cells of the gastric mucosa as the inactive pro-enzyme pepsinogen, which contains a 44 amino acid autoinhibitory domain. The proenzyme is packaged into granules and stored inside the gastric mucosal cells. When food is consumed, neuronal signals traveling along the vagus nerve trigger secretion of HCl and pepsinogen into the stomach. In the highly acidic environment of the stomach, pepsinogen unfolds and undergoes autocatalytic cleavage, forming the active protease pepsin. Infection by the bacterium H. pylori induces hypersecretion of both stomach acid and pepsin, contributing to the formation of gastric and duodenal ulcers. In addition, excessive amounts of pepsin can play a role in the degradation of the esophageal and laryngeal tissue observed in chronic gastroesophageal reflux disease. While the majority of pepsinogen is expressed in the gastric mucosa, a small amount of the inactive enzyme is also released into the blood. Serum pepsinogen levels correlate with functional changes to gastric mucosa and are often measured for the screening of gastric cancer and chronic H. pylori-induced gastritis. BioVision’s Pepsin Assay Kit is a homogenous assay that allows for quantification of pepsin activity in gastric tissue and various biological fluids. The assay utilizes a synthetic peptide substrate bearing both a fluorophore and a fluorescence quencher. Upon cleavage by pepsin, the fluorophore-bearing peptide fragment is unquenched to produce a bright fluorescent signal (Ex/Em = 328/418 nm). Lysosomal aspartic proteases in the peptidase A1 family (Cathepsin D and E) do not interfere with the assay. The assay is rapid, simple to perform and is vastly more sensitive than the classical hemoglobin degradation assay, with a detection limit of 500 μU pepsin activity per well
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Pepsin/Pepsinogen   Activity   Kit   Fluorometric  
• Short name
  Pepsin/Pepsinogen Activity Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Pepsin/Pepsinogen Activity test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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