PCR-Legionella spp Plus Detection Kit

• Catalog number
  K1451-96
• Price
  793.67 USD
• Size
  96 Rxns

• Description
  Legionella spp & Legionella pneumophila detection kit.
• Detection Method
  Real-time PCR method
• Species Reactivity
  Legionella spp and/or Legionella pneumophila
• Applications
  An ideal tool for an fast and reliable Amplification and Detection of specific DNA fragments from Legionella spp and/or Legionella pneumophila by the real-time PCR method (Multiplex PCR).
• Sample Type
  Water sample
• Features Benefits
  Fast: results in less than 24 hours with minimal handling., Inclusivity: Primers and probes have been tested satisfactorily in 14 strains of L. pneumophila and 30 species of the genus Legionella., Amplification limit: Legionella spp: 5 UG per reaction. L. pneumophila: 100 UG per reaction., Detection: probe labelled with fluorescent dyes– Legionella: FAM-BHQ1a; L. pneumophila: HEXBHQ1; IAC:CY5-BHQ2., Quantification limit: Legionella spp: 50 UG/L. L. pneumophila: 1000 UG/L., Exclusivity: No cross-reactions with other frequent bacterial agents have been observed., Simultaneously detect and differentiate Legionella spp. and L. pneumophila in the same tube, Reliable and highly specific Results., Thermal cycler: Agilent Mx3005P, Applied Biosystems 7300, 7500 and other cyclers, Quantification Dynamic range: 5 logs, Easy and Ready-to-use Kit.
• Storage Conditions
  -20°C
• Shipping Conditions
  Dry Ice
• Shelf life
  12 months
• Background
  PCR-Legionella spp Plus Detection Kit (Legionella spp & Legionella pneumophila) is an ideal tool for an fast and reliable Amplification and Detection of specific DNA fragments from Legionella spp and/or Legionella pneumophila by the real-time PCR method (Multiplex PCR). The Kit includes all reagents required in a comfortable ready-to-use Multiplex PCR MasterMix. The optimized MasterMix contains a Buffer, dNTPs, Hot-start DNA Polymerase, DNA-free water, mgCl2 and an Internal Amplification Control (IAC) whose detection indicates the absence of PCR inhibitors. Primers and Probes for the amplification of IAC as well as for the amplification of the target gene are included in the MasterMix. The probe for the detection of target gene is labelled with the FAM (Legionella spp) and HEX fluorochrome (L. pneumophila), whereas the probe for the detection of IAC is labelled with the CY5 fluorochrome. In addition, the kit includes both Positive Control and Negative Control. The Positive control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a Negative control reaction should be included every time the kit is used. Include DNAready Lysis Buffer to extract the DNA from the sample prior to PCR Detection.
• Data Sheet Link
• Additional description
  The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.
• Group
  PCR, polymerase chain reaction
• About
  TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
• Properties
  Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.

• Gene target
  Legionella   spp   Plus   Kit  
• Short name
  PCR-Legionella spp Plus Kit
• Technique
  detection, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
• Alternative name
  PCR test kit-Legionella spp Plus quantification reagent
• Alternative technique
  kits, dna-amplification
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Disease
  legionella

MeSH Data

• Name
  Nucleic Acid Amplification Techniques
• Concept
  Scope note: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
• Tree numbers
  • E05.393.620
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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