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PCR-Legionella spp Detection Kit

  • Catalog number
    K1450-96
  • Product name
    PCR-Legionella spp Detection Kit
  • Size
    96 Rxns
  • Description
    Legionella spp Detection Kit
  • Detection Method
    PCR
  • Species Reactivity
    Legionella spp
  • Applications
    An ideal tool for a fast and reliable Amplification and Detection of specific DNA fragment from Legionella spp by the real-time PCR method.
  • Sample Type
    Bacteria, Legionella spp, Environmental and Biological Samples (water, sediments, sputum, blood, serum, and urine samples)., Air conditioners and water supply systems
  • Features Benefits
    Reliable and highly specific Results., Exclusivity: 100%. Primers and probes have been tested satisfactorily in 28 non-Legionella bacteria., Simple and Fast: results in less than 24 hours with minimal handling., Amplification limit: 2.5 copies/reaction (95%)., Thermal cycler: Agilent Mx3005P, Applied Biosystems 7300, 7500 and other cyclers., Detection: probe labelled with fluorescent dyes– Legionella: FAM-TAMRA; IAC: JOE-TAMRA, Quantification limit: 5 copies/reaction (95%)., Easy and Ready-to-use Kit., Inclusivity: 100%. Primers and probes have been tested satisfactorily in 29 strains of Legionella, Quantification Dynamic range: 8 logs
  • Storage Conditions
    -20°C
  • Shipping Conditions
    Dry Ice
  • Shelf life
    12 months
  • Background
    Legionella spp Detection Kit is an ideal tool for a fast and reliable Amplification and Detection of specific DNA fragment from Legionella spp by the real-time PCR method. The Kit includes all reagents required in a comfortable ready-to-use PCR Master Mix. The optimized Master Mix contains a Buffer, dNTPs, Hot-start DNA Polymerase, DNA-free water, mgCl2 and an Internal Amplification Control (IAC) whose detection indicates the absence of PCR inhibitors. Primers and Probes for the amplification of IAC as well as for the amplification of the target gene are included in the Master Mix. The Probe for the detection of Target Gene is labelled with the FAM (Legionella spp), whereas the probe for the detection of IAC is labelled with the JOE fluorochrome. In addition, the kit includes both Positive Control and Negative Control. The Positive Control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a Negative control reaction should be included every time the kit is used. Include DNA ready Lysis Buffer to extract the DNA from the sample prior to PCR Detection.
  • Data Sheet Link
    Data_Sheet_Link
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
  • Gene target
    Legionella spp Kit
  • Short name
    PCR-Legionella spp Kit
  • technique filter
    • detection
    • PCR
  • Technique
    detection, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
  • Alternative technique
    kits, dna-amplification
  • Disease
    legionella
  • disease filter
    • legionella