PCR-Legionella spp Detection Kit

• Catalog number
  K1450-96
• Price
  Please ask
• Size
  96 Rxns

• Description
  Legionella spp Detection Kit
• Detection Method
  PCR
• Species Reactivity
  Legionella spp
• Applications
  An ideal tool for a fast and reliable Amplification and Detection of specific DNA fragment from Legionella spp by the real-time PCR method.
• Sample Type
  Bacteria, Legionella spp, Environmental and Biological Samples (water, sediments, sputum, blood, serum, and urine samples)., Air conditioners and water supply systems
• Features Benefits
  Reliable and highly specific Results., Exclusivity: 100%. Primers and probes have been tested satisfactorily in 28 non-Legionella bacteria., Simple and Fast: results in less than 24 hours with minimal handling., Amplification limit: 2.5 copies/reaction (95%)., Thermal cycler: Agilent Mx3005P, Applied Biosystems 7300, 7500 and other cyclers., Detection: probe labelled with fluorescent dyes– Legionella: FAM-TAMRA; IAC: JOE-TAMRA, Quantification limit: 5 copies/reaction (95%)., Easy and Ready-to-use Kit., Inclusivity: 100%. Primers and probes have been tested satisfactorily in 29 strains of Legionella, Quantification Dynamic range: 8 logs
• Storage Conditions
  -20°C
• Shipping Conditions
  Dry Ice
• Shelf life
  12 months
• Background
  Legionella spp Detection Kit is an ideal tool for a fast and reliable Amplification and Detection of specific DNA fragment from Legionella spp by the real-time PCR method. The Kit includes all reagents required in a comfortable ready-to-use PCR Master Mix. The optimized Master Mix contains a Buffer, dNTPs, Hot-start DNA Polymerase, DNA-free water, mgCl2 and an Internal Amplification Control (IAC) whose detection indicates the absence of PCR inhibitors. Primers and Probes for the amplification of IAC as well as for the amplification of the target gene are included in the Master Mix. The Probe for the detection of Target Gene is labelled with the FAM (Legionella spp), whereas the probe for the detection of IAC is labelled with the JOE fluorochrome. In addition, the kit includes both Positive Control and Negative Control. The Positive Control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a Negative control reaction should be included every time the kit is used. Include DNA ready Lysis Buffer to extract the DNA from the sample prior to PCR Detection.
• Data Sheet Link
• Additional description
  The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.
• Group
  PCR, polymerase chain reaction
• About
  TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
• Properties
  Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.

• Gene target
  Legionella   spp   Kit  
• Short name
  PCR-Legionella spp Kit
• Technique
  detection, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
• Alternative name
  PCR test kit-Legionella spp quantification reagent
• Alternative technique
  kits, dna-amplification
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Disease
  legionella

MeSH Data

• Name
  Polymerase Chain Reaction
• Concept
  Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
• Tree numbers
  • E05.393.620.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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