Neutrophil Elastase Activity Assay kit (Fluorometric)

• Catalog number
  K383-100
• Price
  Please ask
• Size
  100 assays

• Description
  Validated using blood samples
• Summary
  • Detection method- Fluorescence (Ex/Em = 380/500 nm) • Applications- Determine activity of purified Neutrophil Elastase - Detect the activity of Neutrophil Elastase in plasma, blood - Screen/study/characterize chemotactic agents causing Neutrophil stimulation and release of Elastase
• Detection Method
  Fluorescence (Ex/Em = 380/500 nm)
• Applications
  • Determine activity of purified Neutrophil Elastase • Detect the activity of Neutrophil Elastase in plasma, blood • Screen/study/characterize chemotactic agents causing Neutrophil stimulation and release of Elastase
• Sample Type
  • Purified enzyme • Blood, plasma
• Features Benefits
  • Simple & convenient protocol. • Can detect neutrophil elastase activity as low as 1 ng in a variety of samples. • Kit contains all necessary reagents for measuring neutrophil elastase activity.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Neutrophil Elastase (NE, EC 3.4.21.37, leukocyte elastase, elastase 2, or serine elastase) is a cytotoxic serine protease. It is stored in the azurophil granules of neutrophil granulocyte and is released following cell stimulation, e.g. by pathogens, immune complexes or chemotactic agents (PMA). When the extracellular NE concentration exceeds the buffering capacity of endogenous inhibitors, it causes degradation of a wide range of extracellular matrix proteins, including fibronectin, laminin, proteoglycans, collagens, and elastin. Extracellular elastase is implicated in the signs, symptoms and disease progression of inflammatory lung disorders (such as cystic fibrosis, COPD, lung emphysema) via its role in the inflammatory processes, mucus overproduction and lung tissue damage. BioVision's Neutrophil Elastase Activity Assay Kit utilizes the ability of NE to proteolytically cleave a synthetic substrate and release a fluorophore, AFC, which can be easily quantified by fluorescence microplate readers. This assay kit is simple, rapid and can detect as low as 1 ng of Neutrophil Elastase in a variety of samples.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Neutrophil   Elastase   Activity   kit   Fluorometric  
• Short name
  Neutrophil Elastase Activity Assay kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Neutrophil Elastase Activity test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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