Neprilysin Activity Assay Kit (Fluorometric)

• Catalog number
  K487-100
• Price
  Please ask
• Size
  100 assays

• Description
  High-throughput compatible, quick and easy screening forNEP ligands; Includes human recombinant NEP
• Summary
  • Detection method- Fluorometric (Ex/Em = 330/430 nm) • Application-NEP Activity Assay is highly sensitive with a detection limit of approximately 20 μU/ mg
• Detection Method
  Fluorometric (Ex/Em = 330/430 nm)
• Species Reactivity
  Eukaryotes
• Applications
  NEP Activity Assay is highly sensitive with a detection limit of approximately 20 μU/ mg
• Sample Type
  • Tissue homogenates: lung, kidney, etc. • Cell culture: adherent or suspension cells • Purified enzyme
• Features Benefits
  • Simple and reliable test NEP activity in biological samples • High-throughput compatible • Includes NEP positive control
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Neprilysin (NEP, EC 3.4.24.11), also known as neutral endopeptidase, enkephalinase, CD10, and common acute lymphoblastic leukemia antigen, is a zinc-containing transmembrane metalloproteinase. It is able to hydrolyze very important endogenous peptides, such as natriuretic atrial factor, enkephalins, substance P, bradykinin and amyloid β (Aβ) peptide. Thus, NEP is a potentially therapeutic target in important pathological conditions such as cardiovascular disease, prostate cancer, and Alzheimer’s disease. NEP has also been used as a biological marker of a type of child leukemia and the detection of NEP in endometrial stromal cells had been proposed as a helpful tool in diagnosis of endometriosis. NEP is currently a focus of major interest in cardiovascular and neurological research. BioVision’s Neprilysin Activity Kit utilizes the ability of an active NEP to cleave a synthetic substrate (Abz-based peptide) to release a free fluorophore. The released Abz can be easily quantified using a fluorescence microplate reader. The substrate is specific to NEP and can differentiate the NEP activity from Trypsin and other structurally similar zinc metalloproteinase in biological samples such as Angiotensin-Converting Enzymes (ACE1, ACE2), Endothelin Converting Enzymes (ECE1, ECE2). Our assay kit is simple, specific and can detect as low as 20 µU/ mg of NEP activity.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Neprilysin   Activity   Kit   Fluorometric  
• Short name
  Neprilysin Activity Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Neprilysin Activity test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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