NADH Oxidase Activity Assay Kit (Fluorometric)

• Catalog number
  K2019-100
• Price
  Please ask
• Size
  100 assays

• Description
  to measure NADH oxidase activity
• Summary
  BioVision’s NADH Oxidase Activity Assay Kit couples oxidation of NADH by NOX and reduction of a colorless probe to a brightly colored product generating fluorescence at Ex/Em = 535/587 nm. The fluorescence generated is directly proportional to the NOX activity in samples. The kit can be used to determine NOX activity in a variety of sample type with a detection limit of ~ 15 µU of NOX activity per reaction.
• Detection Method
  Fluorescence (Ex/Em = 535/587 nm)
• Species Reactivity
  Mammalian
• Applications
  Analysis of ROS in different samples, Mechanistic studies of various cancers, Measurement of NADH oxidase activity in various tissues/cells, Screening anti-cancer drugs
• Sample Type
  Cell culture: adherent or suspension cells, Animal tissues: Liver, muscle, heart etc.
• Features Benefits
  Simple, Rapid, Can be used to determine NOX activity in a variety of sample type with a detection limit of ~ 15 µU of NOX activity per reaction.
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  NADH oxidase (Nicotinamide Adenine Dinucleotide Phosphate Oxidase, NOX) is a homotetrameric flavoenzyme that catalyzes the oxidation of NADH by molecular oxygen to yield NAD+. NOX is one of the well-known cancer biomarkers and is shown to be stimulated by growth factors and hormones. Several studies revealed the high activity of NADH oxidase in cancer patients as compared to healthy volunteers. More importantly, inhibition of NOX correlates with reduced cancer cell growth and induction of apoptosis. RNA interference targeting NOX expression in cancer cells effectively restored the non-cancerous phenotypes thereby further supporting the vital role of NOX in cancer progression. Furthermore, NADH oxidase is critical for the regeneration of NAD+, for use in glycolysis, and for the reduction of oxygen thereby preventing the formation of damaging reactive oxygen species. This makes NADH oxidase an attractive therapeutic target. BioVision’s NADH Oxidase Activity Assay Kit couples oxidation of NADH by NOX and reduction of a colorless probe to a brightly colored product generating fluorescence at Ex/Em = 535/587 nm. The fluorescence generated is directly proportional to the NOX activity in samples. The kit can be used to determine NOX activity in a variety of sample type with a detection limit of ~ 15 µU of NOX activity per reaction.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  NADH   Oxidase   Activity   Kit   Fluorometric  
• Short name
  NADH Oxidase Activity Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  NADH Oxidase Activity test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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