Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric)
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Catalog number
K561-100
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Price
Please ask
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Size
100 assays
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Description
Industry's most sensitive kit to measure and quantitate MAGL enzymatic activity in biological samples
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Summary
• Detection method - Fluorescence (Ex/Em = 360/460 nm) • Applications- • Measurement of monoacylglycerol lipase activity in various tissues/cells.
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Detection Method
Fluorometric (Ex/Em = 360/460 nm)
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Species Reactivity
Mammalian
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Applications
• Measurement of monoacylglycerol lipase activity in various tissues/cells.
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Sample Type
• Animal tissues: brain, intestine etc. • Cell culture: Adherent or suspension cells
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Features Benefits
• Simple one-step reaction • Easy Sample preparation; assay only 1-2 hrs • Non-radiometric fluorescent detection • HTP adaptable
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Monoacylglycerol Lipase (MAGL, E.C. 3.1.1.23) is an important enzyme for regulation of endocannabinoid signaling in human physiology. MAGL is a serine hydrolase that generates free fatty acid and glycerol from monoacylglycerols such as 2-arachidonoyl glycerol (2-AG), one of the principal endocannabinoids active in the nervous system. One free fatty acid that is released by MAGL activity, arachidonic acid, is a precursor for eicosanoids, a family of pro-inflammatory signaling molecules. The leftover glycerol moiety can also serve various purposes as a metabolic building block and energy source. Inhibition or inactivation of MAGL leads to 2-AG buildup, and this has myriad biological consequences. Antinociceptive effects, anti-inflammatory and neuroprotective effects in Parkinson’s and Alzheimer’s diseases as well as reduced addiction withdrawal symptoms are some of the outcomes of MAGL inhibition. In addition, the downstream eicosanoid signaling pathway is affected; this pathway plays a role in diverse physiological processes including inflammation, immunity, nociception and blood pressure. As a central node in the regulation of both lipid signaling and energy mobilization, MAGL is of considerable interest as a therapeutic target. BioVision’s Monoacylglycerol Lipase activity assay kit provides a quick, sensitive and easy way for measuring MAGL activity in various samples. In this assay, a fluorescent substrate is cleaved to generate arachidonic acid and fluorescent metabolite and the increased fluorescence is measured at Ex/Em 360/460 nm. To identify the signal generated specifically by MAGL, a specific inhibitor is included that allows the user to differentiate MAGL activity from other sources of fluorescence. The assay is simple to perform, high-throughput adaptable and can detect as low as 0.1 mU of MAGL activity
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Gene symbol
MGLL
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Short name
Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric)
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Technique
Assay, assays
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Host
Assay
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Alternative name
Monoacylglycerol Lipase (MAGL) Activity test reagent (fluorescence)
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
monoglyceride lipase
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2001-12-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Product images
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