Maltose Phosphorylase Activity Kit (Fluorometric)

  • Catalog number
    K353-100
  • Price
    Please ask
  • Size
    100 assays
  • Description
    for detecting Maltose Phosphorylase activity in different bacterial lysates
  • Summary
    BioVision’s Maltose Phosphorylase Activity Assay Kit is the first commercial assay to determine the Maltose Phosphorylase activity in different bacterial lysates. The assay converts maltose to produce Glucose, which is then detected by a set of enzymatic reactions to generate a fluorescent product with a Ex/Em 535/587 nm. The fluorescence signal is directly proportional to the Maltose Phosphorylase activity. BioVision’s Matlose Phosphorylase Activity Assay Kit is rapid, sensitive and a convenient tool for detecting Maltose Phosphorylase activity. It can detect as low as 0.5 mU under the assay conditions.
  • Detection Method
    Fluorescence (Ex/Em 535/587 nm)
  • Species Reactivity
    Bacterial lysates
  • Applications
    Analysis of Maltose Phosphorylase kinetics in inhibition or activation screening, Measurement of Maltose Phosphorylase activity in different bacteria
  • Sample Type
    Bacterial lysates
  • Features Benefits
    Sensitive, Rapid, Convenient tool for detecting Maltose Phosphorylase activity, It can detect as low as 0.5 mU under the assay conditions
  • Storage Conditions
    -20°C
  • Shipping Conditions
    Gel Pack
  • Shelf life
    12 months
  • Background
    Maltose phosphorylase (EC 2.4.1.8) belongs to the family of hexosyltransferases. It catalyzes maltose and inorganic phosphate to produce glucose and glucose-1-phosphate (G1P) during Maltose metabolism. Maltose phosphorylase is commonly identified in gram positive and gram negative bacteria including E. coli, Bacillus subtilis, Enterococcus sp. and Lactobacillus brevis etc. BioVision’s Maltose Phosphorylase Activity Assay Kit is the first commercial assay to determine the Maltose Phosphorylase activity in different bacterial lysates. The assay converts maltose to produce Glucose, which is then detected by a set of enzymatic reactions to generate a fluorescent product with a Ex/Em 535/587 nm. The fluorescence signal is directly proportional to the Maltose Phosphorylase activity. BioVision’s Matlose Phosphorylase Activity Assay Kit is rapid, sensitive and a convenient tool for detecting Maltose Phosphorylase activity. It can detect as low as 0.5 mU under the assay conditions.
  • Gene target
  • Short name
    Maltose Phosphorylase Activity Kit (Fluorometric)
  • Alternative name
    Maltose Phosphorylase Activity reagent (fluorescence)
  • Alternative technique
    kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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