Lysyl Oxidase Activity Assay Kit (Fluorometric)

• Catalog number
  K928-100
• Price
  Please ask
• Size
  100 assays

• Description
  Simple, sensitive, enzymatic assay to measure Lysyl Oxidase Activity in biological samples. Only kit in the market including active positive control
• Summary
  • Detection method - Flurometric (535/587 nm) • Species reactivity - Mammalians • Applications - Measurement of Lysyl Oxidase activity in various biological samples, and purified proteins • Includes active lysyl oxidase
• Detection Method
  Fluorometric (Ex/Em = 535/587 nm)
• Species Reactivity
  Mammalian
• Applications
  • Measurement of Lysyl Oxidase activity in various biological samples and protein preparations
• Sample Type
  • Purified protein, cell media
• Features Benefits
  • Simple, highly sensitive, high-throughput compatible • Includes active Lysyl Oxidase
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Lysyl oxidase is a cell-secreted amine oxidase that catalyzes the final step of collagen and elastin cross-linking. As a result, lysyl oxidase is required for the biosynthesis of functional extracellular matrices. Inhibition of lysyl oxidase can result in osteolathyrism, a condition characterized by fragile connective tissue and paralysis. Recent work has also suggested lysyl oxidase to play an important role in cancer development: Lysyl oxidase promotes tumor growth and progression in vivo, cancer cell invasion, and premetastatic niche formation. BioVision’s Lysyl Oxidase Activity Fluorometric Assay Kit allows for quantitative evaluation of lysyl oxidase activity of purified enzyme and its inhibitors as well as secreted endogenous enzyme in cell media. Enzyme activity is detected upon oxidation of substrate, which results in release of reaction intermediates. Reaction intermediates are subsequently detected by BioVision’s LOX Probe, resulting in fluorescent signal that can be measured at Ex/Em= 535/587 nm. Each kit includes a positive control as well as a specific lysyl oxidase inhibitor that allows the user to correct each sample for any non-specific signal. Lysyl Oxidase Activity Fluorometric Assay Kit can detect as little as 40 ng of recombinant enzyme in vitro.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Lysyl   Oxidase   Activity   Kit   Fluorometric  
• Short name
  Lysyl Oxidase Activity Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Lysyl Oxidase Activity test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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