Hydroxy Acid Oxidase Activity Assay Kit (Fluorometric)
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Catalog number
K2010-100
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Price
Please ask
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Size
100 assays
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Description
for measuring HAO activity in biological samples
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Summary
Hydroxy Acid Oxidase (HAO) (EC 1.1.3.15), also known as glycolate oxidase is a flavin mononucleotide dependent enzyme that oxidizes alpha hydroxy acid to oxo acid. Glycolate is the primary substrate for HAO, which gets oxidized to glyoxalate. HAO contributes to the general pathway of fatty acid α-oxidation and plays an important role in ethylene glycol poisoning. It is expressed in liver, kidney and pancreas. The most well studied enzyme of this family is the plant glycolate oxidase that has an essential role in photorespiration. BioVision’s Hydroxy Acid Oxidase Activity Kit is a simple plate-based fluorometric assay for measuring HAO activity in biological samples. HAO oxidizes hydroxy carboxylate, which is accompanied by the release of H2O2. H2O2 then oxidizes a non-fluorescent probe to a fluorescent product in the presence of enzyme mix via an enzymatic reaction. The assay can detect as low as 1 µU of HAO activity in biological samples.
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Detection Method
Fluorescence (Ex/Em = 535/587 nm)
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Species Reactivity
All
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Applications
Measurement of HAO activity
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Sample Type
Purified protein, Cell lysate, Recombinant enzyme, Plant tissue lysate, Animal tissue lysate (Liver tissue)
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Features Benefits
The assay can detect as low as 1 µU of HAO activity in biological samples., Simple plate-based fluorometric assay for measuring HAO activity in biological samples.
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Storage Conditions
-20°C
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Shipping Conditions
Gel Pack
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Shelf life
12 months
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Background
Hydroxy Acid Oxidase (HAO) (EC 1.1.3.15), also known as glycolate oxidase is a flavin mononucleotide dependent enzyme that oxidizes alpha hydroxy acid to oxo acid. Glycolate is the primary substrate for HAO, which gets oxidized to glyoxalate. HAO contributes to the general pathway of fatty acid α-oxidation and plays an important role in ethylene glycol poisoning. It is expressed in liver, kidney and pancreas. The most well studied enzyme of this family is the plant glycolate oxidase that has an essential role in photorespiration. BioVision’s Hydroxy Acid Oxidase Activity Kit is a simple plate-based fluorometric assay for measuring HAO activity in biological samples. HAO oxidizes hydroxy carboxylate, which is accompanied by the release of H₂O₂. H₂O₂ then oxidizes a non-fluorescent probe to a fluorescent product in the presence of enzyme mix via an enzymatic reaction. The assay can detect as low as 1 µU of HAO activity in biological samples.
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Short name
Hydroxy Acid Oxidase Activity Assay Kit (Fluorometric)
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Technique
Assay, assays
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Host
Assay
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Alternative name
Hydroxy Acid Oxidase Activity test reagent (fluorescence)
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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