HDAC6 Activity Assay Kit (Fluorometric)
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Catalog number
K466-100
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Price
Please ask
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Size
100 assays
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Description
High-throughput compatible, quick and easy screening for HDAC6 activity in biological samples; Includes human recombinant HDAC6 positive control
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Summary
• Detection method - Fluorescence (Ex/Em 380/490 nm) • Species reactivity - Eukaryotes • Applications - Measurement of HDAC6 activity in various biological samples.
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Detection Method
Fluorescence (Ex/Em 380/490 nm)
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Species Reactivity
Eukaryotes
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Applications
• Rapid, high-throughput screening of drugs and novel ligands. • Development of structure-activity relationship (SAR) models to predict HDAC6 interaction liability of novel compounds.
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Sample Type
Samples containing drugs, inhibitors or ligands (compounds that can interact and affect HDAC6 activity)
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Features Benefits
• Simple, highly sensitive, high-throughput compatible • Rapid screening of HDAC6 inhibitors • Kit includes Tubacin, an HDAC6 inhibitor, and a stable, recombinant human HDAC6
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Storage Conditions
-80°C
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Shipping Conditions
dry ice
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Shelf life
12 months
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Background
Histone Deacetylase 6 (HDAC6, EC 3.5.1.98), is a class IIb HDAC enzyme that deacetylates an ε-N-acetyl lysine of histone and non-histone protein substrates. It is a unique member of HDAC family as it contains two deacetylase domains that are proposed to function independent of each other. HDAC6 can shuttle between the nucleus and cytoplasm, suggesting potential extra-nuclear functions by regulating the acetylation status of non-histone substrates. HDAC6 also affects transcription and translation by regulating the heat-shock protein 90 (Hsp90) and stress granules. Elevated HDAC6 activity is associated with cell motility and increases α-tubulin deacetylation, thus influencing cancer cell metastasis. In addition, mutations in HDAC6 gene have been associated with Alzheimer's disease. HDAC6-selective inhibitors are considered as promising targets for autoimmune, oncology and inflammatory diseases. BioVision’s HDAC6 Activity Assay Kit utilizes deacetylase activity of HDAC6 towards a synthetic acetylated-peptide substrate resulting in the release of an AFC fluorophore, which can be easily quantified using a conventional microplate reader. This simple and high-throughput adaptable assay kit can be used to detect HDAC6 activity (as low as 3 U) in complex biological samples.
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Gene symbol
HDAC6
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Short name
HDAC6 Activity Assay Kit (Fluorometric)
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Technique
Assay, assays
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Host
Assay
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Alternative name
histone deacetylase 6 Activity test reagent (fluorescence)
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
histone deacetylase 6
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2000-11-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Histone deacetylases, class IIB
- Protein phosphatase 1 regulatory subunits
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VEGA ID
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Locus Specific Databases
Product images
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