HDAC Activity Fluorometric Assay Kit
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Catalog number
K330-100
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Price
Please ask
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Size
100 assays
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Description
Two step assay, HTS
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Summary
• Detection method- Fluorescence (Ex/Em 350-380/440-460 nm) • Species reactivity- Mammalian • Applications- Well suited for high throughput applications. HDAC inhibitors and substrates and related antibodies are also available separately.
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Sample Type
Cell lysate, nuclear extract
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Features Benefits
• Simple two-step procedure; takes around than 1 hour • Fast and convenient • The assay method eliminates radioactivity/extractions/ and/or chromatography as used in the traditional assays.
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Inhibition of histone deacetylase (HDAC) has been implicated to modulate transcription and induce apoptosis or differentiation in cancer cells. However, screening compounds for HDAC inhibition has been difficult due to the lack of convenient tools for analyzing HDAC activity. The Fluorometric HDAC Activity Assay Kit provides a fast and fluorescence-based method that eliminates radioactivity, extractions, or chromatography, as used in traditional assays. The new procedure requires only two easy steps, both performed on the same microtiter plate. First, the HDAC substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (e.g., HeLa nuclear extract). Deacetylation of the substrate sensitizes the substrate, so that further treatment with the Lysine Developer produces a fluorophore. The fluorophore can be easily analyzed using a fluorescence plate reader or a fluorometer. The assay is well suited for high throughput screening applications. HDAC inhibitors and antibodies are also available separately.
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Gene symbol
HDAC4, HDAC9
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Short name
HDAC Activity Fluorometric Assay Kit
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Technique
Assay, assays
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Host
Assay
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Alternative name
HDAC Activity fluorescence test reagent
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
histone deacetylase 4
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Synonyms gene
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Synonyms gene name
- brachydactyly-mental retardation syndrome
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Synonyms
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GenBank acession
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Locus
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Discovery year
2000-11-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Histone deacetylases, class IIA
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
histone deacetylase 9
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Synonyms
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GenBank acession
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Locus
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Discovery year
2002-09-06
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Entrez gene record
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Pubmed identfication
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Classification
- Histone deacetylases, class IIA
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VEGA ID
Product images
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