Glutamine Synthetase Activity Assay Kit (Colorimetric)

• Catalog number
  K2056-100
• Price
  Please ask
• Size
  100 assays

• Description
  For measuring GS activity in biological samples
• Summary
  Glutamine Synthetase Activity Assay Kit is a simple, plate-based colorimetric assay for measuring GS activity in biological samples. In this assay, GS hydrolyzes Glutamate to Glutamine and ADP. ADP in the subsequent enzymatic reaction, in presence of ADP Converter, ADP Developer Mix and ADP Probe forms a colorimetric product that is measured at OD 570 nm. The assay is simple, sensitive and can detect as low as 10 µU of GS in biological samples.
• Detection Method
  Absorbance at 570 nm
• Species Reactivity
  Mammalian
• Applications
  Measurement of Glutamine Synthetase activity in tissue and cell lysate
• Sample Type
  Cell Lysates, i.e. 3T3 cell lysates,Tissue lysates, i.e. Brain tissue
• Features Benefits
  The assay is simple, sensitive and can detect as low as 10 µU of GS in biological samples.,A simple, plate-based colorimetric assay for measuring GS activity in biological samples.
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Glutamine Synthetase (GS, Glutamate-Ammonia Ligase; EC 6.3.1.2) is a mitochondrial enzyme that catalyzes the ATP-dependent ligation of glutamate and ammonia to glutamine. GS is ubiquitously expressed in human tissues and plays a critical role in detoxifying ammonia and regulating the concentration of glutamate or glutamine. It is also a key enzyme in nitrogen metabolism and a principal source for protein and nucleic acid synthesis. Additionally, GS activity has been linked to several neurological disorders such as Alzheimer’s disease, schizophrenia, hepatic encephalopathy and epilepsy. Furthermore, two mutations of human GS (R324C and R341C) have been linked to congenital glutamine deficiency with severe brain malformations resulting in multi-organ failure and neonatal death. BioVision’s Glutamine Synthetase Activity Assay Kit is a simple, plate-based colorimetric assay for measuring GS activity in biological samples. In this assay, GS hydrolyzes Glutamate to Glutamine and ADP. ADP in the subsequent enzymatic reaction, in presence of ADP Converter, ADP Developer Mix and ADP Probe forms a colorimetric product that is measured at OD 570 nm. The assay is simple, sensitive and can detect as low as 10 µU of GS in biological samples.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Glutamine   Synthetase   Activity   Kit  
• Short name
  Glutamine Synthetase Activity Assay Kit (Colorimetric)
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Glutamine Synthetase Activity test reagent (colorimetry)
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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