Succinyl-CoA Synthetase Activity Colorimetric Assay Kit

• Catalog number
  K597-100
• Price
  Please ask
• Size
  100 assays

• Description
  Only kit on the market to measure Succinyl CoA Synthatase Activity
• Summary
  • Detection method- Absorbance (OD 450 nm). • Applications- • Measurement of Succinyl-CoA Synthetase activity in various tissues/cells • Analysis of cell signaling pathway
• Detection Method
  Absorbance (OD 450 nm).
• Applications
  • Measurement of Succinyl-CoA Synthetase activity in various tissues/cells • Analysis of cell signaling pathway
• Sample Type
  • Animal tissues: heart, liver, muscle, etc. • Purified mitochondria • Cell culture: adherent or suspension cells
• Features Benefits
  • Simple & Rapid Protocol • High-Throughput adaptable • This kit can detect less than 0.1 mU of Succinyl-CoA Synthetase activity in a variety of samples.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Succinyl-CoA Synthetase (SCS, also called Succinyl-CoA ligase, Succinate Thiokinase) (EC 6.2.1.5) is a critical enzyme in the citric acid cycle and an important metabolic intermediate for porphyrin, heme and ketone body biosynthesis. It is located in the mitochondrial matrix and is a heterodimer composed of one α and one β subunit. In humans, Succinyl-CoA Synthetase deficiency causes the build-up of lactic acid leading to lactic acidosis, which can be fatal in infants. Measurement and analysis of SCS activity is useful for both mechanistic studies as well as for diagnostic purposes. In BioVision’s Succinyl-CoA Synthetase Activity Assay, SCS converts succinate into succinyl-CoA in the presence of ATP and CoA. Succinyl-CoA reacts with the Developer to form a colored product with strong absorbance at 450 nm. This assay kit is simple, sensitive, and high-throughput adaptable. It can detect less than 0.1 mU of Succinyl-CoA Synthetase activity in a variety of samples.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Succinyl-CoA   Synthetase   Activity   Kit  
• Short name
  Succinyl-CoA Synthetase Activity Colorimetric Assay Kit
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Succinyl-CoA Synthetase Activity colorimetry test reagent
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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