Glutamine Synthetase Inhibitor Screening Kit (Fluorometric)

• Catalog number
  K2059-100
• Price
  Please ask
• Size
  100 assays

• Description
  For the screening human Glutamine Synthetase inhibitors
• Summary
  BioVision’s Glutamine Synthetase Inhibitor Screening Kit is a plate-based fluorometric assay for the screening human GS inhibitors. In this kit, the activity of GS is monitored by the formation of a fluorescent product formed via an enzymatic reaction and is detected at Ex/Em = 535/587 nm. But in the presence of GS inhibitors, the GS enzyme loses its activity, which results in the decrease of fluorescence intensity.
• Detection Method
  Fluorescence Ex/Em = 535/587 nm
• Species Reactivity
  Human
• Applications
  Screening of potential inhibitors of Human Glutamine Synthetase.
• Features Benefits
  A plate-based fluorometric assay for the screening human GS inhibitors.,The assay is simple and easy to use.
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Glutamine Synthetase (GS; EC 6.3.1.2) is a key enzyme that regulates nitrogen metabolism by catalyzing the formation of glutamine from glutamate and ammonia. It is ubiquitously expressed in humans and is linked to several neurological disorders including Alzheimer’s disease, schizophrenia, epilepsy etc. Inhibition of GS in animal models have shown significant therapeutic benefits in hepatic encephalopathy, amyotrophic lateral sclerosis, inflammatory liver failure, cancer and other human diseases. Additionally, GS is highly expressed in bacteria and is essential for its growth. Inhibition of Mycobacterium tuberculosis GS has been used as a therapeutic strategy against Tuberculosis. BioVision’s Glutamine Synthetase Inhibitor Screening Kit is a plate-based fluorometric assay for the screening human GS inhibitors. In this kit, the activity of GS is monitored by the formation of a fluorescent product formed via an enzymatic reaction and is detected at Ex/Em = 535/587 nm. But in the presence of GS inhibitors, the GS enzyme loses its activity, which results in the decrease of fluorescence intensity. Methionine Sulfoximine (MSO), a well-characterized inhibitor of prokaryotic and eukaryotic GSs with different Ki values is used as the positive control. The assay is simple and can used to identify and characterize human GS inhibitors.
• Data Sheet Link
• Additional description
  Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.

• Gene target
  Glutamine   Synthetase   Inhibitor   Screening   Kit   Fluorometric  
• Short name
  Glutamine Synthetase Inhibitor Screening Kit (Fluorometric)
• Alternative name
  Glutamine Synthetase suppressor detection reagent (fluorescence)
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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