Glutamate Carboxypeptidase II Inhibitor Screening Kit (Fluorometric)

• Catalog number
  K440-100
• Price
  Please ask
• Size
  100 assays

• Description
  Convenient assay for screening potential GCPII inhibitors
• Summary
  Glutamate Carboxypeptidase II Inhibitor Screening Kit (Fluorometric): Simple method to screen/study/characterize GCPII Inhibitors, includes 2-PMPA. Convenient & Reliable. 100 Assays.
• Detection Method
  Fluorometric (Ex/Em= 535/587 nm)
• Species Reactivity
  All
• Applications
  Screening/characterizing Glutamate Carboxypeptidase II inhibitors
• Features Benefits
  Includes Inhibitor Control (2-PMPA), Simple and reliable test to screen GCPII inhibitors/activators, High-throughput compatible
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Glutamate Carboxypeptidase II [GCPII, EC 3.4.17.21; N-acetylated-alpha-linked acidic dipeptidase (NAALADase), prostate-specific membrane antigen (PSMA) or folate hydrolase (FOLH1)] is a multifunctional zinc-metallopeptidase. Within the last decade, GCPII has been studied as a promising pharmacological target for the treatment of various pathological disorders and diseases and tremendous efforts have been made in order to discover potent GCPII inhibitors. NAALADase, the brain isozyme, plays an important role in Glutamate biosynthesis and could be used for treating neuronal damage caused by excess glutamate in brain. For example, inhibition of GCPII has shown neuroprotective characteristics in animal models suffering several acute and chronic conditions (stroke, neuropathic and inflammatory pain, schizophrenia, depression or amyotrophic lateral sclerosis). In males, PSMA is overexpressed in patients suffering prostate cancer and thus, it is used as a diagnostic marker for this disease. BioVision’s Glutamate Carboxypeptidase II Inhibitor Screening Kit is an in vitro tool in order to screen potential human GCPII inhibitors. The provided substrate is transaminated in the presence of GCPII producing glutamate. The detection system is based on an enzymatic reaction in which a fluorogenic probe is reduced generating a stable signal. In the presence of 2-PMPA, a potent GCPII Inhibitor, the enzymatic activity is arrested, thus generating lower fluorometric signal. The assay kit is a simple plate-based format and provides a rapid and reliable test for high-throughput screening of GCPII inhibitors.
• Data Sheet Link
• Additional description
  Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.

• Gene target
  Glutamate   Carboxypeptidase   Inhibitor   Screening   Kit   Fluorometric  
• Short name
  Glutamate Carboxypeptidase II Inhibitor Screening Kit (Fluorometric)
• Alternative name
  Glutamate Carboxypeptidase II suppressor detection reagent (fluorescence)
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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