Neprilysin Inhibitor Screening Kit (Fluorometric)

• Catalog number
  K996-100
• Price
  Please ask
• Size
  100 assays

• Description
  High-throughput compatible, quick and easy screening for Neprilysin inhibitors; Includes human Neprilysin.
• Summary
  • Detection method- Fluorometric (Ex/Em = 320/420 nm) • Application-Screening/characterizing/studying Neprilysin inhibitors/activators
• Detection Method
  Fluorometric (Ex/Em = 320/420 nm)
• Species Reactivity
  Human
• Applications
  Screening/characterizing/studying Neprilysin inhibitors/activators
• Features Benefits
  • Simple and reliable test to screen Neprilysin inhibitors/activators • High-throughput compatible • Includes Inhibitor Control (Thiorpan)
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Neprilysin (NEP, EC 3.4.24.11), also known as neutral endopeptidase, enkephalinase, CD10, and common acute lymphoblastic leukemia antigen, is a type II zinc-containing transmembrane metalloproteinase. It is able to hydrolyze very important endogenous peptides, such as natriuretic atrial factor, enkephalins, substance P, bradykinin and amyloid beta-peptide. Thus, NEP is a potentially therapeutic target in important pathological conditions such as cardiovascular disease, prostate cancer, Alzheimer’s disease and analgesia. NEP has also been used as a biological marker of a type of childhood leukemia called CALLA. NEP is currently a focus of interest in cardiovascular and neurobiological research. NEP inhibitors have shown promising results as potential treatment alternative for some conditions including hypertension, heart failure, renal failure, and improvement of β-cell function in obese type 2 diabetes mellitus. The combination of NEP inhibitor and angiotensin II type 1 receptor blockade has been used clinically to treat patients with chronic heart failure. BioVision’s NEP Inhibitor Screening Kit can be used to screen for potential inhibitors of NEP activity. It utilizes the ability of an active NEP to cleave a synthetic MCA based peptide substrate to release a free fluorophore. The released MCA can be easily quantified using a fluorescence microplate reader. In the presence of an NEP specific inhibitor, the enzyme loses its activity which results in decrease of fluorescence intensity. The assay kit is simple and can be used to identify and characterize NEP inhibitors in a high-throughput format
• Data Sheet Link
• Additional description
  Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.

• Gene target
  Neprilysin   Inhibitor   Screening   Kit   Fluorometric  
• Short name
  Neprilysin Inhibitor Screening Kit (Fluorometric)
• Alternative name
  Neprilysin suppressor detection reagent (fluorescence)
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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