Collagenase Inhibitor Screening Kit (Fluorometric)

• Catalog number
  K833-100
• Price
  Please ask
• Size
  100 assays

• Description
  Simple, quick, fluorometric assay to screen/characterize Collagenase Inhibitors.
• Summary
  A simple and quick assay to fluorometrically (Ex/Em = 490/520 nm, 515 nm cut-off) screen/study/characterize potential inhibitors of Collagenase.
• Detection Method
  Fluorescence (Ex/Em = 490/520 nm)
• Species Reactivity
  N/A
• Applications
  ·    Screening/studying/characterizing potential Collagenase inhibitors
• Sample Type
  Chemical libraries, small molecules
• Features Benefits
  Simple and quick protocol Detect using a fluorometer or fluorescence microplate reader.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Collagenase (EC 3.4.24.3) is an enzyme in the matrix metalloproteinase (MMP) family that breaks down collagen, assisting in degradation of the extracellular matrix, a key step in the pathogenesis of bacteria and tumor cell invasion. Collagen is an abundant structural protein present in the connective tissue of animals. Collagenase has been used clinically for the treatment of Dupuytren’s contracture, an affliction characterized by a thickening of connective tissue. BioVision’s Collagenase Inhibitor Screening Kit provides a quick and sensitive way for screening, studying and characterizing potential inhibitors of Collagenase. The kit uses Self-Quenched BODIPY conjugate of Gelatin (Type B) as a fluorogenic substrate to monitor the activity of Collagenase. Gelatin consists of a heterogeneous mixture of proteins of high average molecular weight derived from collagen Upon proteolytic digestion of the highly quenched BODIPY-labeled gelatin by Collagenase, the de-quenched BODIPY yields bright green fluorescence that can be assayed using a fluorometer or a fluorescence microplate reader (Ex/Em: 490 nm / 520 nm, 515 nm cutoff). In the presence of a Collagenase inhibitor, the gelatin is not digested, the dequenching of BODIPY does not occur and the fluorescent signal is not produced.
• Data Sheet Link
• Additional description
  Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.

• Gene target
  Collagenase   Inhibitor   Screening   Kit   Fluorometric  
• Short name
  Collagenase Inhibitor Screening Kit (Fluorometric)
• Alternative name
  Collagenase suppressor detection reagent (fluorescence)
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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