EZCut™ SUMO Protease 1 (GST-tagged), Yeast Recombinant
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Catalog number
7869-100
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Price
Please ask
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Size
100 Units
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Synonyms
Ulp1 peptidase, SUMO Protease
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Alternative_names
Ulp1 peptidase, SUMO Protease
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Description
>90% Pure, Active, Yeast Recombinant, GST-Tagged SUMO Protease 1, to cleave the SUMO tag from recombinant proteins.
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥90%
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Assay
SDS-PAGE
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Activity Specifications test method
The EZCut™ SUMO Protease 1 (GST-tagged) has an activity of ≥10,000 units/mg.
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Results
≥10,000 units/mg
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Unit Definition
One unit is defined as the amount of EZCut™ SUMO Protease 1 required to cleave >90% of 5 µg a control protein substrate (SUMO-GFP) in 1 h at 37°C.
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Molecular Weight
52.6 kDa (403-621 aa + N-terminal GST).
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Storage Temp
-80°C
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Shipping
Dry ice
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Shelf Life
12 months
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Concentration
1 mg/ml
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Appearance
Liquid
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Physical form description
1 mg/ml solution in 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT and 20% glycerol pH 8.0
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Background Information
SUMO (Small Ubiquitin-like MOdifiers) Protease 1 (Ulp1, Ubl-specific protease 1 from Saccharomyces cerevisiae) is a highly active cysteine protease. It is highly specific as it recognizes the tertiary structure of the ubiquitin-like (UBL) protein, SUMO (Smt3), rather than its amino acid sequence. SUMO fusion tag, as an N-terminal fusion partner, has been shown to enhance functional protein production in prokaryotic and eukaryotic expression systems with significantly improved protein stability and solubility. BioVision’s Recombinant EZCut™ SUMO Protease 1 containing an N-terminal GST-tag is expressed in E. coli and purified by proprietary chromatographic techniques. The EZCut™ SUMO protease 1 can be used to cleave SUMO protein tag from recombinant SUMO-fusion proteins. The optimal temperature for cleavage is 30°C; however, the enzyme is active over wide ranges of temperature and pH. After the completion of the cleavage reaction, the protease can be easily removed from the reaction by affinity chromatography using the Glutathione resin (Cat. # 6555-1,10,50).
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Amino acid sequence
403-621 aa + N-terminal GST
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
MGST3, SUMO1
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Short name
EZCut™ SUMO Protease 1 (GST-tagged), Yeast Recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Host
Yeast, Ascomycota
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Species
Yeasts
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Alternative name
EZCut™ SUMO Protease 1 (GST-tagged), Yeast Rec.
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Alternative technique
rec
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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