EZCut™ SUMO Protease 1 (His-tagged), Yeast Recombinant
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Catalog number7868-2500
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Price:
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Size2500 Units
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SynonymsUlp1 peptidase, SUMO Protease
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Alternative_namesUlp1 peptidase, SUMO Protease
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Description>90% Pure, Active, Yeast Recombinant, His-Tagged SUMO Protease 1, to cleave the SUMO tag from recombinant proteins.
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥90%
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AssaySDS-PAGE
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Activity Specifications test methodThe EZCut™ SUMO Protease 1 (His-tagged) has an activity of ≥ 1 X 106 units/mg.
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Biological activityThe EZCut™ SUMO Protease 1 (His-tagged) has an activity of ≥ 1 X 106 units/mg.
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Results≥ 1 X 106 units/mg.
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Unit DefinitionOne unit is defined as the amount of EZCut™ SUMO Protease 1 required to cleave >90% of 5 µg a control protein substrate (SUMO-GFP) in 1 h at 37°C.
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Molecular Weight28.7 kDa (403-621 aa + N-terminal Poly-His tag).
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Storage Temp-80°C
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ShippingDry ice
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Shelf Life12 months
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Concentration0.1 mg/ml (100 U/µl)
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AppearanceLiquid
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Physical form description0.1 mg/ml (100 U/µl) solution in 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT and 20% glycerol pH 8.0
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Background InformationSUMO (Small Ubiquitin-like MOdifiers) Protease 1 (Ulp1, Ubl-specific protease 1 from Saccharomyces cerevisiae) is a highly active cysteine protease. It is highly specific as it recognizes the tertiary structure of the ubiquitin-like (UBL) protein, SUMO (Smt3), rather than its amino acid sequence. SUMO fusion tag, as an N-terminal fusion partner, has been shown to enhance functional protein production in prokaryotic and eukaryotic expression systems with significantly improved protein stability and solubility. BioVision’s Recombinant EZCut™ SUMO Protease 1 containing an N-terminal His-tag is expressed in E. coli and purified by proprietary chromatographic techniques. The EZCut™ SUMO Protease 1 can be used to cleave SUMO protein tag from recombinant SUMO-fusion proteins. The optimal temperature for cleavage is 30°C; however, the enzyme is active over wide ranges of temperature and pH. After the completion of the cleavage reaction, the protease can be easily removed from the reaction by affinity chromatography using the Ni chelating resin (Cat. # 6562-1, 10, 100, 500).
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Amino acid sequence403-621 aa + N-terminal Poly-His tag
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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Conjugationhistidine
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolSUMO1
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Short nameEZCut™ SUMO Protease 1 (His-tagged), Yeast Recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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HostYeast, Ascomycota
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Labelhis-tagged
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SpeciesYeasts
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Alternative nameEZCut™ SUMO Protease 1 (histidine-tagged), Yeast Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene namesmall ubiquitin like modifier 1
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Synonyms gene
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Synonyms gene name
- ubiquitin-like 1 (sentrin)
- SMT3 suppressor of mif two 3 homolog 1 (yeast)
- SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-06-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data