EZClickTM Fucose (FucAz) Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)

• Catalog number
  K592-100
• Price
  Please ask
• Size
  100 assays

• Summary
  • Detection method- Flow Cytometry (Ex/Em 488/(530/590) nm) and Fluorescence Microscopy • Sample type- Adherent and suspension cells • Species reactivity- Mammalian • Applications- This assay provides a convenient and accurate procedure to measure glycoproteins (Fucosylation) in biological samples.
• Detection Method
  Flow Cytometry (Ex/Em 480/(530/590) nm) and Fluorescence Microscopy
• Species Reactivity
  Mammalian
• Applications
  This assay provides a convenient and accurate procedure to measure glycoproteins (Fucosylation) in biological samples
• Sample Type
  Adherent and suspension cells
• Features Benefits
  Simple, fast, does not require lengthy incubation times
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Glycans are vital components of glycoproteins, glycolipids, and proteoglycans in all domains of life. Glycosylation occurs co- or post-translationally on >50% of eukaryotic proteins resulting in membrane-associated, intracellular, or secreted glycoproteins that are crucial in cellular processes, protein bioactivity and metabolic turnover. Glycoproteins are grouped by the type of carbohydrate and amino acid linkage site. Fucose is found in glycoproteins and glycolipids present in vertebrates, invertebrates, plants, and bacteria. It is often a terminal sugar in glycans participating in cell–cell signaling and migration implicated in physiological and pathological processes such as fertilization, embryogenesis, lymphocyte trafficking, immune responses, and cancer metastasis. Most target proteins undergoing fucosylation are secretory or membrane proteins on the cell surface. Fucosylation is one of the most important modifications involved in cancer and inflammation. Several types of biomarkers containing fucose are linked to cancer e.g. alpha-fetoprotein (AFP) is used in the diagnosis of hepatocellular carcinoma and surface glycosphingolipid Globo H is an epitope found on the cell surface of breast, prostate, and ovarian cancers. Since glycoproteins are not directly encoded in the genome, methods of their characterization and analyses are of great interest. Thus, BioVision offers EZClickTM Fucose (FucAz) Modified Glycoprotein Assay Kit, a highly specific, simple and robust method for labeling and detection of fucosylated proteins within cells. We use fucose analog that is fed directly into the cells, processed via fucose salvage pathway and incorporated into the glycoproteins. Followed by click reaction with alkyne-containing dye, this system offers a powerful method for imaging the localization, trafficking, and dynamics of glycans, or detection by FACS for quantitative studies. Fucose-labeled glycoproteins can be directly detected in 1D or 2D gels using the appropriate excitation sources, or enriched by immunoprecipitation with biotin-alkyne or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1. Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.

• Gene target
  EZClickTM   Fucose   FucAz   Modified   Glycoprotein   Kit   FACS/Microscopy  
• Short name
  EZClickTM Fucose (FucAz) Modified Glycoprotein Assay Kit (FACS/Microscopy, )
• Technique
  Assay, FACS, assays
• Host
  Assay
• Label
  green
• Alternative name
  EZClickTM Fucose (FucAz) Modified Glycoprotein test reagent (flow cytometer cell sorting/Microscopy, Green Fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Microscopy
• Concept
  Scope note: The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
• Tree numbers
  • E01.370.350.515
  • E05.595
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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