EZClick™ O-GalNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)

• Catalog number
  K560-100
• Price
  Please ask
• Size
  100 assays

• Summary
  • Detection method- Flow Cytometry (Ex/Em 488/(530/590) nm) and Fluorescence Microscopy • Sample type- Adherent and suspension cells • Species reactivity- Mammalian • Applications- This assay provides a convenient and accurate procedure to measure glycoproteins (O-GalNAc Modified Proteins) in biological samples.
• Detection Method
  Flow Cytometry (Ex/Em 480/(530/590) nm) and Fluorescence Microscopy
• Species Reactivity
  Mammalian
• Applications
  This assay provides a convenient and accurate procedure to measure glycoproteins (O-GalNAc Modified Proteins) in biological samples
• Sample Type
  Adherent and suspension cells
• Features Benefits
  Simple, fast, does not require lengthy incubation times
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Glycans are vital components of glycoproteins, glycolipids, and proteoglycans in all domains of life. Glycoproteins are grouped by the type of carbohydrate and amino acid linkage site. N-linked glycosylation is a modification of asparagine, whereas O-linked glycosylation occurs through the hydroxyl group of serine and threonine residues. Glycosylation occurs co- or post-translationally on >50% of eukaryotic proteins resulting in membrane-associated, intracellular, or secreted glycoproteins that are crucial in cellular processes, protein bioactivity and metabolic turnover. Attachment of O-linked mucin-type glycans is a common post-translational modification initiated by the addition of N-acetyl-galactosamine (GalNAc) to a Ser or Thr residue of newly assembled protein. Resulting GalNAc-(Ser/Thr) structure (Tn-antigen) can be further modified by the addition of either a sialic acid residue (sialyl-Tn), GlcNAc to form GlcNAc-GalNAc (core 3), or a galactose residue to form the GalGalNAc (core 1) structures respectively. The core 3 and 1structures are then further extended to form long and branched complex O-glycans. The core 1 (TF or T antigen) acts like oncofetal antigen, overexpressed in cancerous and precancerous conditions due to the Golgi apparatus disorder. GalNAc linked to the first mannose of glycosylphosphatidylinositol (GPI) core has been previously reported to be heterogeneously present on mammalian GPI-anchored proteins. Thus BioVision offers EZClickTM O-GalNAc Modified Glycoprotein Assay Kit, a highly specific, simple and robust method for labeling and detection of O-GalNAc-glycosylated proteins within cells. We use a modified galactosamine precursor that is fed directly into the cells, processed by the GalNAc salvage pathway to form the intermediate uridine diphospho (UDP)-GalNAz, which is recognized by GalNAc transferases in the Golgi and incorporated into the protein. Followed by click reaction with alkyne-containing dye, this system offers a powerful method for imaging the localization, trafficking, and dynamics of glycans, or detection by FACS for quantitative studies. Labeled Glycoproteins can be directly detected in 1D or 2D gels using the appropriate excitation sources, or enriched by immunoprecipitation with biotin-alkyne or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1. Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.

• Gene target
  EZClick™   O-GalNAc   Modified   Glycoprotein   Kit   FACS/Microscopy  
• Short name
  EZClick™ O-GalNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, )
• Technique
  Assay, FACS, assays
• Host
  Assay
• Label
  green
• Alternative name
  EZClick™ O-GalNAc Modified Glycoprotein test reagent (flow cytometer cell sorting/Microscopy, Green Fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Microscopy
• Concept
  Scope note: The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
• Tree numbers
  • E01.370.350.515
  • E05.595
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products