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Available ordering format
Inquire
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Immunogen
highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212
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Raised in
Chicken
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Total IgY in 10 mM TRIS, 0.15 mM NaCl, pH 8 with 0.02 % sodium azide
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
store at 4°C; make aliquots to avoid working with a stock. Or store in small aliquots at -20°C. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
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Verified applications
ELISA (ELISA), Western blot (WB)
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Connected products
AS15 2987 | GFP (affinity purified), rabbit antibodyAS15 3000 | GFP (protein A purified), rabbit antibodyAS15 2999 | GFP (total IgG), rabbit antibodyAS15 3001 | GFP, peroxidase conjugated, rabbit antibodyAS15 3002 | GFP (purified recombinant protein)other tag antibodies
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Recommended dilutions for use
1: 5000 - 1: 25 000 (ELISA), 1: 2000 - 1: 10 000 (WB)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
native GFP, recombinant GFP (E.coli), all variants of GFP
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
Minimal cross-reactivity with E.coli proteins
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References
to be added when available, antibody released in September 2015
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Scientific context
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
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Notes
None
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Protein number
P42212
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TAIR number
Refer to NCBI
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Test
Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
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Properties
Chicken yolk polyclonal Immunoglobulin Y (abbreviated as IgY) is a type of immunoglobulin which is the major antibody in bird, reptile, and lungfish blood. It is also found in high concentrations in chicken egg yolk. As with the other immunoglobulins, IgY is a class of proteins which are formed by the immune system in reaction to certain foreign substances, and specifically recognize them.
