Cytidine Deaminase Activity Assay Kit (Fluorometric)

• Catalog number
  K451-100
• Price
  Please ask
• Size
  100 assays

• Description
  Rapid & simple assay to measures total Cytidine deaminase activity
• Summary
  • Detection method- Fluorescence (Ex/Em = 410/470 nm) • Application- Detection of Cytidine Deaminase activity
• Detection Method
  Fluorescence (Ex/Em = 410/470nm)
• Species Reactivity
  Eukaryotes
• Applications
  Measurement of CDA activity in various tissues/cells Evaluation of CDA activity for comparison of activity rates in normal versus tumor tissues
• Sample Type
  • Cell and tissue lysate • Nuclear Extract • Cell Medium • Purified recombinant protein
• Features Benefits
  • Rapid, and convenient • The kit measures total Activity of Cytidine Deaminase with limit of quantification 1 µU recombinant Cytidine Deaminase. Range of the assay: 10-100 µU.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Cytidine Deaminase (3.5.4.5) is a homotetramer enzyme that catalyzes the irreversible deamination of cytidine to produce uridine, thus maintaining the intracellular pyrimidine pool of uridine. Cytidine as well as adenosine, thymidine, guanosine and uridine are one of the five standard nucleosides with which nucleic acids are composed. Nucleosides accumulate or are recycled from salvage pathways within the cell and play an important role in DNA and RNA synthesis and subsequent cell division. Tumorigenesis is intricately linked to metabolism of macromolecular precursors, specifically, the building blocks of DNA and RNA. Thus tumor cells have greater concentrations of uridine and cytidine analogues (402 µM +/- 252) compared to normal cells (83 µM +/- 133), to support the increased demand for nucleotides in mitosis in actively dividing cells. BioVision’s Cytidine Deaminase Activity Assay Kit uses CDA to convert cytidine to the uridine and NH3, as intermediates. The intermediate products then react with a proprietary Reaction Mix to generate a stable fluorophore that can be detected fluorometrically (Ex/Em= 410/470 nm). The kit is suitable for measuring CDA activity as low as 1 µUnit (1 pmole/min/well) in biological samples
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Cytidine   Deaminase   Activity   Kit   Fluorometric  
• Short name
  Cytidine Deaminase Activity Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Cytidine Deaminase Activity test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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