Chymotrypsin Activity Assay Kit (Fluorometric)

#

• Catalog number
  K352-100
• Price:

  496.89 USD

  Ask for price
• Size
  100 assays

# #

• Summary
  • Detection method- Fluorometric (380/460 nm) • Species reactivity- Mammalian • Application- The assay can detect as low as 0.01 mU of Chymotrypsin in various samples.
• Detection Method
  Fluorescence (Ex/Em= 380/460 nm)
• Species Reactivity
  Mammalian
• Applications
  Measurement of chymotrypsin activity in tissues / cultured cells.
• Sample Type
  Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, etc.
• Features Benefits
  • Simple procedure; takes ~ 1-2 hours • Fast and convenient
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Chymotrypsin (EC 3.4.21.1) is a key serine protease involved in dietary protein digestion in mammals. It is primarily produced by the pancreas, but may be expressed in other tissues, including spleen and liver. In the pancreas, chymotrypsin is initially expressed as the inactive proenzyme chymotrypsinogen, which is cleaved by other proteases to chymotrypsin, its active form. Chymotrypsin specifically cleaves peptide bonds at the C-terminal end of bulky hydrophobic or aromatic amino acids (such as tyrosine, tryptophan or phenylalanine). BioVision’s Chymotrypsin Activity Assay kit uses a synthetic fluorogenic substrate, enabling kinetic measurement of chymotrypsin activity in cell and tissue lysates. A chymotrypsin activator cleaves chymotrypsinogen to form active chymotrypsin, which then hydrolyzes the non-fluorescent substrate to release a stable fluorophore (Ex/Em = 380/460 nm). The kit includes a selective chymotrypsin inhibitor that can be used to measure specific chymotrypsin activity in samples containing non-specific proteases and endopeptidases that may also metabolize the substrate. The assay can detect as low as 0.01 mU of Chymotrypsin
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

#

• Gene target
  Chymotrypsin   Activity   Kit   Fluorometric  
• Short name
  Chymotrypsin Activity Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Chymotrypsin Activity test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Product images

Similar products