cAMP Phosphodiesterase Activity Assay Kit (Fluorometric)

• Catalog number
  K2013-100
• Price
  Please ask
• Size
  100 assays

• Description
  to measure cPDE activity in various sample types
• Summary
  Cyclic adenosine-3’,5’-monophosphate (cyclic AMP or cAMP) is a vital second messenger molecule in eukaryotes as well as a relevant signaling effector in bacteria. Intracellular cAMP-mediated signaling cascades control many biological pathways such as glucose and lipid metabolism via activation of protein kinase A and cyclic nucleotide gated ion channels. Intracellular cAMP is synthesized from ATP by the enzyme adenylyl cyclase and is inactivated by hydrolytic cyclic-AMP phosphodiesterase enzymes (cPDEs; EC 3.1.4.53). Inhibition of cPDEs has been shown to impact many biological pathways including cognition, neuronal signaling, inflammation and vascular smooth muscle contractility. Eleven superfamilies of PDEs with varying selectivity for cAMP or other cyclic nucleotides have been identified in mammals. The predominant phosphodiesterase isoforms involved in the degradation of cAMP are phosphodiesterase 4 (PDE4) family members. There are many unique isoforms of the enzyme, expressed either as products of different genes or through alternative splicing, and their expression levels are cell-type dependent. BioVision’s cAMP Phosphodiesterase Activity Assay Kit provides a rapid, sensitive and straightforward way to measure cPDE activity in various sample types. In this assay, AMP produced by cPDE activity is metabolized by the enzyme mix, developer mix and converter mix to generate an intermediate compound, which reacts with a probe, yielding a fluorescent signal that can be measured at Ex/Em = 535/587 nm. This assay can detect cPDE activity as low as 0.1 µU per well.
• Detection Method
  Fluorescence (Ex/Em = 535/587 nm)
• Species Reactivity
  All
• Applications
  Measurement of Cyclic-AMP Phosphodiesterase activity in various tissues or cells
• Sample Type
  Adherent or suspension cultured cells, Animal tissues: liver, lung, intestine, etc., Purified enzyme preparations
• Features Benefits
  Sensitive, Rapid, Straightforward way to measure cPDE activity in various sample types
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Cyclic adenosine-3’,5’-monophosphate (cyclic AMP or cAMP) is a vital second messenger molecule in eukaryotes as well as a relevant signaling effector in bacteria. Intracellular cAMP-mediated signaling cascades control many biological pathways such as glucose and lipid metabolism via activation of protein kinase A and cyclic nucleotide gated ion channels. Intracellular cAMP is synthesized from ATP by the enzyme adenylyl cyclase and is inactivated by hydrolytic cyclic-AMP phosphodiesterase enzymes (cPDEs; EC 3.1.4.53). Inhibition of cPDEs has been shown to impact many biological pathways including cognition, neuronal signaling, inflammation and vascular smooth muscle contractility. Eleven superfamilies of PDEs with varying selectivity for cAMP or other cyclic nucleotides have been identified in mammals. The predominant phosphodiesterase isoforms involved in the degradation of cAMP are phosphodiesterase 4 (PDE4) family members. There are many unique isoforms of the enzyme, expressed either as products of different genes or through alternative splicing, and their expression levels are cell-type dependent. BioVision’s cAMP Phosphodiesterase Activity Assay Kit provides a rapid, sensitive and straightforward way to measure cPDE activity in various sample types. In this assay, AMP produced by cPDE activity is metabolized by the enzyme mix, developer mix and converter mix to generate an intermediate compound, which reacts with a probe, yielding a fluorescent signal that can be measured at Ex/Em = 535/587 nm. This assay can detect cPDE activity as low as 0.1 µU per well.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  cAMP   Phosphodiesterase   Activity   Kit   Fluorometric  
• Short name
  cAMP Phosphodiesterase Activity Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  cathelicidin antimicrobial peptide Phosphodiesterase Activity test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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