ApoSENSOR™ ATP Cell Viability Bioluminescence Assay Kit
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Catalog number
K254-1000
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Price
Please ask
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Size
1000 assays
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Description
Ultra-sensitive Assay, HTS
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Summary
• Detection method- Luminometer or Beta Counter. • Species reactivity- Mammalian • Kit size- 200, 1000 assays • Applications- Bioluminescent detection of the ATP level via luciferase catalyzed reaction for a rapid screening of apoptosis and cell viability in mammalian cells.
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Sample Type
Cell and tissue lysates, culture media, urine, soil, sludge, plasma and serum, as well as many other biological fluids
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Features Benefits
• Simple one-step procedure; takes only 30 minutes • Fast and convenient • The assay can be done directly in culture plates requiring no harvest/washing/or sample preparations. The assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 mammalian cells/well).
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Cell death (especially apoptosis) is an energy-dependent process that requires ATP. As ATP levels fall to a point where the cell can no longer perform basic metabolic functions, the cell will die. A typical apoptotic cell exhibits a significant decrease in ATP level. Therefore, loss of ATP level in cell has been used as an indicator of cell death. In contrast, cell proliferation has been recognized by increased levels of ATP. The ApoSENSOR™ Cell Viability Assay Kit utilizes bioluminescent detection of the ATP levels for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or Beta Counter. The assay can be fully automatic for high throughput (10 seconds/sample) and is extremely sensitive (detects 10-100 mammalian cells/well). The high sensitivity of this assay has led to many other applications for detecting ATP production in various enzymatic reactions, as well as for detecting low level bacterial contamination in samples such as blood, milk, urine, soil, and sludge.
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Test
Biovision supplies other types of Assays as 1.
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Additional description
For cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbol
VPS9D1, NFATC2
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Short name
ApoSENSOR™ ATP Viability Bioluminescence Assay Kit
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Technique
Assay, assays
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Host
Assay
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Alternative name
ApoSENSOR™ adenosine triphosphate cellular cytotoxity Bioluminescence test reagent
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
cell
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Gene info
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Identity
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Gene
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Long gene name
VPS9 domain containing 1
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Synonyms gene
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Synonyms gene name
- chromosome 16 open reading frame 7
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Synonyms
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GenBank acession
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Locus
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Discovery year
2000-09-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
Product images
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