ADP/ATP Ratio Bioluminescence Assay Kit, ApoSENSOR
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Catalog numberK255-200
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Price:
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Size200 assays
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DescriptionSensitive Assay, HTS
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Summary• Detection method- Luminometer or Beta Counter. • Species reactivity- Mammalian • Applications- Bioluminescent detection of the ADP and ATP levels for a rapid screening of apoptosis, necrosis, growth arrest, and cell proliferation simultaneously in mammalian cells.
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Sample TypeCell and tissue lysates, culture media, urine, soil, sludge, plasma and serum, as well as many other biological fluids
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Features Benefits• Simple one-step procedure; takes only 30 minutes • Fast and convenient • The ADP/ATP ratio assay offers highly consistent results and with excellent correlation to other apoptosis markers (e.g. TUNEL-based assays and caspase assays). In addition the assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 cells/well).
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Storage Conditions-20°C
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Shipping Conditionsgel pack
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Shelf life12 months
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BackgroundThe changes in ADP/ATP ratio have been used to differentiate the different modes of cell death and viability. Increased levels of ATP and decreased levels of ADP have been recognized in proliferating cells. In contrast, decreased levels of ATP and increased levels of ADP are recognized in apoptotic cells. The decrease in ATP and increase in ADP are much more pronounced in necrosis than apoptosis. The ApoSENSORTM ADP/ATP Ratio Assay kit utilizes bioluminescent detection of the ADP and ATP levels for a rapid screening of apoptosis, necrosis, growth arrest, and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or Beta Counter. ADP level is measured by its conversion to ATP that is subsequently detected using the same reaction. The assay can be fully automatic for high throughput and is highly sensitive (detects 100 mammalian cells/well).
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TestBiovision supplies other types of Assays as 1.
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Gene target
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Gene symbolWDTC1, ADPGK, VPS9D1, NFATC2
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Short nameADP/ATP Ratio Bioluminescence Assay Kit, ApoSENSOR
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TechniqueAssay, assays
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HostAssay
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Alternative nameADP/adenosine triphosphate Ratio Bioluminescence test reagent, ApoSENSOR
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Alternative techniquearrays, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene nameWD and tetratricopeptide repeats 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-12-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- WD repeat domain containing
- Tetratricopeptide repeat domain containing
- DDB1 and CUL4 associated factors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameADP dependent glucokinase
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-09-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameVPS9 domain containing 1
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Synonyms gene
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Synonyms gene name
- chromosome 16 open reading frame 7
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-09-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- VPS9 domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenuclear factor of activated T cells 2
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Synonyms gene name
- nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
- nuclear factor of activated T-cells 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-11-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- IPT domain containing
- Nuclear factors of activated T-cells
- MicroRNA protein coding host genes
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VEGA ID