ADP/ATP Ratio Bioluminescence Assay Kit, ApoSENSOR
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Catalog number
K255-200
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Price
Please ask
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Size
200 assays
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Description
Sensitive Assay, HTS
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Summary
• Detection method- Luminometer or Beta Counter. • Species reactivity- Mammalian • Applications- Bioluminescent detection of the ADP and ATP levels for a rapid screening of apoptosis, necrosis, growth arrest, and cell proliferation simultaneously in mammalian cells.
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Sample Type
Cell and tissue lysates, culture media, urine, soil, sludge, plasma and serum, as well as many other biological fluids
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Features Benefits
• Simple one-step procedure; takes only 30 minutes • Fast and convenient • The ADP/ATP ratio assay offers highly consistent results and with excellent correlation to other apoptosis markers (e.g. TUNEL-based assays and caspase assays). In addition the assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 cells/well).
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
The changes in ADP/ATP ratio have been used to differentiate the different modes of cell death and viability. Increased levels of ATP and decreased levels of ADP have been recognized in proliferating cells. In contrast, decreased levels of ATP and increased levels of ADP are recognized in apoptotic cells. The decrease in ATP and increase in ADP are much more pronounced in necrosis than apoptosis. The ApoSENSORTM ADP/ATP Ratio Assay kit utilizes bioluminescent detection of the ADP and ATP levels for a rapid screening of apoptosis, necrosis, growth arrest, and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or Beta Counter. ADP level is measured by its conversion to ATP that is subsequently detected using the same reaction. The assay can be fully automatic for high throughput and is highly sensitive (detects 100 mammalian cells/well).
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Gene symbol
WDTC1, ADPGK, VPS9D1, NFATC2
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Short name
ADP/ATP Ratio Bioluminescence Assay Kit, ApoSENSOR
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Technique
Assay, assays
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Host
Assay
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Alternative name
ADP/adenosine triphosphate Ratio Bioluminescence test reagent, ApoSENSOR
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Gene info
Gene info
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Identity
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Gene
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Long gene name
VPS9 domain containing 1
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Synonyms gene
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Synonyms gene name
- chromosome 16 open reading frame 7
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Synonyms
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GenBank acession
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Locus
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Discovery year
2000-09-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
Product images
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