StayBrite™ Highly Stable ATP Bioluminescence Assay Kit
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Catalog numberK791-1000
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Price:
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Size1000 assays
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DescriptionHighly Sensitive, Stable Assay, HTS
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Summary• Detection method- Luminescence • Species reactivity- Mammalian • Kit size- Convenient sizes (100 and1000 assays) • Application- The activity profile shows a linear detection range for ATP values as low as 10 fmol/assay (1 nM).
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Sample TypeCell and tissue culture homogenates
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Features Benefits• Simple procedure; takes ~ 20-30 minutes • Fast and convenient • Stable for >10 hours at room temperature and >60 minutes at 37°C. • The stable reagent provides more accuracy than any other ATP assay kits.
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Storage Conditions-20°C
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Shipping Conditionsgel pack
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Shelf life12 months
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BackgroundAdenosine-5’-triphosphate (ATP) plays an important role in living systems. It has also been used as an indicator to monitor many biological processes and disease stages, etc.. An accurate and reliable detection of ATP level has wide applications. Luciferase/Luciferin has been used to sensitively measure ATP levels. Conventional Luciferase/Luciferin ATP detection systems, however, are unstable. Luciferase loses activity rapidly. At BioVision, we have developed a highly stable Luciferase, which is a genetically modified variant derived from the Luciferase of Diaphanes pectinealis (Chinese Firefly) endemic to Yunnan province, China. We named the highly stable luciferase rLucHS. Compared to the normal phenotype of Photinus pyralis, the rLucHS provides enhanced stability, excellent sensitivity, and a broader and more physiologically relevant effective pH range. At all pH’s below ~8.2, rLucHS is significantly more active than Photinus luciferase and is stable for weeks at room temperature and >60 minutes at 37°C. The activity profile shows a linear detection range for ATP values as low as 10 fmol/assay (1 nM). The specific activity of rLucHS is ~ 5x10ᶦᶦRLU/ mg protein. The assay can be fully automated for high throughput (1 second/sample) and is extremely sensitive. The high sensitivity of this assay is ideal for detecting ATP production or consumption in various enzymatic reactions.
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TestBiovision supplies other types of Assays as 1.
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Gene target
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Gene symbolVPS9D1, NFATC2
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Short nameStayBrite™ Highly Stable ATP Bioluminescence Assay Kit
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TechniqueAssay, assays
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HostAssay
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Alternative nameStayBrite™ very Stable adenosine triphosphate Bioluminescence test reagent
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Alternative techniquearrays, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene nameVPS9 domain containing 1
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Synonyms gene
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Synonyms gene name
- chromosome 16 open reading frame 7
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-09-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- VPS9 domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenuclear factor of activated T cells 2
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Synonyms gene name
- nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
- nuclear factor of activated T-cells 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-11-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- IPT domain containing
- Nuclear factors of activated T-cells
- MicroRNA protein coding host genes
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VEGA ID