StayBrite™ Highly Stable ATP Bioluminescence Assay Kit

• Catalog number
  K791-1000
• Price
  Please ask
• Size
  1000 assays

• Description
  Highly Sensitive, Stable Assay, HTS
• Summary
  • Detection method- Luminescence • Species reactivity- Mammalian • Kit size- Convenient sizes (100 and1000 assays) • Application- The activity profile shows a linear detection range for ATP values as low as 10 fmol/assay (1 nM).
• Sample Type
  Cell and tissue culture homogenates
• Features Benefits
  • Simple procedure; takes ~ 20-30 minutes • Fast and convenient • Stable for >10 hours at room temperature and >60 minutes at 37°C. • The stable reagent provides more accuracy than any other ATP assay kits.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Adenosine-5’-triphosphate (ATP) plays an important role in living systems. It has also been used as an indicator to monitor many biological processes and disease stages, etc.. An accurate and reliable detection of ATP level has wide applications. Luciferase/Luciferin has been used to sensitively measure ATP levels. Conventional Luciferase/Luciferin ATP detection systems, however, are unstable. Luciferase loses activity rapidly. At BioVision, we have developed a highly stable Luciferase, which is a genetically modified variant derived from the Luciferase of Diaphanes pectinealis (Chinese Firefly) endemic to Yunnan province, China. We named the highly stable luciferase rLucHS. Compared to the normal phenotype of Photinus pyralis, the rLucHS provides enhanced stability, excellent sensitivity, and a broader and more physiologically relevant effective pH range. At all pH’s below ~8.2, rLucHS is significantly more active than Photinus luciferase and is stable for weeks at room temperature and >60 minutes at 37°C. The activity profile shows a linear detection range for ATP values as low as 10 fmol/assay (1 nM). The specific activity of rLucHS is ~ 5x10ᶦᶦRLU/ mg protein. The assay can be fully automated for high throughput (1 second/sample) and is extremely sensitive. The high sensitivity of this assay is ideal for detecting ATP production or consumption in various enzymatic reactions.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  StayBrite™   Highly   Stable   ATP   Bioluminescence   Kit  
• Gene symbol
  VPS9D1, NFATC2
• Short name
  StayBrite™ Highly Stable ATP Bioluminescence Assay Kit
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  StayBrite™ very Stable adenosine triphosphate Bioluminescence test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:13526
• Gene
  VPS9D1
• Long gene name
  VPS9 domain containing 1
• Synonyms gene
  • C16orf7
• Synonyms gene name
  • chromosome 16 open reading frame 7
• Synonyms
  • ATP-BL
• GenBank acession
  • AB018551
• Locus
  16q24.3
• Discovery year
  2000-09-20
• Entrez gene record
  9605
• Pubmed identfication
  • 10231027
• RefSeq identity
  • NM_004913
• Classification
  • VPS9 domain containing
• VEGA ID
  OTTHUMG00000173219

Gene info

• Identity
  HGNC:7776
• Gene
  NFATC2
• Long gene name
  nuclear factor of activated T cells 2
• Synonyms gene name
  • nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
  • nuclear factor of activated T-cells 2
• Synonyms
  • NF-ATP
  • NFATp
  • NFAT1
• GenBank acession
  • U43342
  • U43341
• Locus
  20q13.2
• Discovery year
  1994-11-16
• Entrez gene record
  4773
• Pubmed identfication
  • 8202141
• RefSeq identity
  • NM_012340
• Classification
  • IPT domain containing
  • Nuclear factors of activated T-cells
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000032747

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