Apolipoprotein E, Human Plasma
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Catalog number
7284-50
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Price
Please ask
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Size
50 ug
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Synonyms
ApoE
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Alternative_names
ApoE
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Description
Apolipoprotein E serves as a ligand for low density receptors.
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Recombinant
No
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Source
Human plasma. Prepared from plasma shown to be non-reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Molecular Weight
34 kDa
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Storage Temp
-80°C
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Shipping
Dry Ice
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Shelf Life
12 months
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Appearance
Frozen
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Physical form description
Frozen in 50 mM NH4HCO3, pH 8.0
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Background Information
Apolipoprotein E serves as a ligand for low density receptors and participates in the transport and redistribution of cholesterol and other lipids. Other functions include immunoregulation and cell growth modulation and differentiation. Apo E is thought to be involved in tissue repair as increased amounts of the protein are found at sites of peripheral nerve injury and regeneration. A mutant form is associated with familial type III hyperlipoproteinemia. The concentration of Apo E in normal plasma is 5 mg per 100 ml. In addition to facilitating solubilization of lipids, these proteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism. Significant quantities of ApoE are produced in liver and brain and to some extent in almost every organ. ApoE is an important constituent of all plasma lipoproteins. It’s interaction with specific ApoE receptor enables uptake of chylomicron remnants by liver cells, which is an essential step during normal lipid metabolism. It also binds with the LDL receptor (apo B/E). Defects in ApoE are a cause of hyperlipoproteinemia type III. ApoE exists in three major isoforms; E2, E3, and E4, which differ from one another by a single amino-acid substitution.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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