Adenosine Deaminase Activity Assay Kit (Fluorometric)

• Catalog number
  K328-100
• Price
  Please ask
• Size
  100 assays

• Description
  Rapid & simple assay to measures total adenosine deaminase activity
• Summary
  • Detection method- Fluorescence (Ex/Em = 535/587 nm) • Application- Detection of Adenosine Deaminase activity
• Detection Method
  Fluorescence (Ex/Em = 535/587 nm)
• Applications
  Detection of Adenosine Deaminase activity
• Sample Type
  • Cell and tissue lysate • Nuclear Extract • Cell Medium • Purified recombinant protein
• Features Benefits
  • Rapid, and convenient • The kit measures total Activity of Adenosine Deaminase with limit of quantification 10 µU recombinant Adenosine Deaminase. Range of the assay: 10-100 µU.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Adenosine Deaminase (ADA) (E.C. 3.5.4.4.) is an enzyme that catalyzes the conversion of adenosine and 2’-deoxyadenosine to inosine and 2’-deoxyinosine. Adenosine Deaminase is widely distributed in various tissues and cells. There are two isoforms, ADA1 and ADA2. ADA1 is widely expressed in most cells in the body, particularly in lymphocytes and macrophages. It is present in the cytosol, nucleus and found associated with dipeptidyl peptidase-4 on the cell membrane. ADA2 was first found in the spleen but is predominantly found in the plasma and serum. Increased serum ADA levels are found in certain infectious diseases such as tuberculosis and various liver diseases such as acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis to name a few. Adenosine Deaminase is also a marker for T-lymphocyte proliferation. In BioVision’s ADA Activity Assay, inosine formed from the breakdown of adenosine is detected via a multi-step reaction, resulting in the formation of an intermediate that reacts with the ADA Probe to generate a fluorescent product. The kit measures total Activity of Adenosine Deaminase with limit of quantification of 10 µU recombinant Adenosine Deaminase. The range of the assay is from 10-100 µU.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Adenosine   Deaminase   Activity   Kit   Fluorometric  
• Short name
  Adenosine Deaminase Activity Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Adenosine Deaminase Activity test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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