MBP(Thr232) Antibody, FITC Conjugated
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Catalog numberbs-5474R-FITC
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Price:
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Size0.1ml
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Long nameMBP(Thr232) Polyclonal Antibody, FITC Conjugated
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CategoryConjugated Primary Antibodies
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ConjugationFITC
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Host OrganismRabbit (Oryctolagus cuniculus)
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Target AntigenMBP Thr232
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SpecificityThis is a highly specific antibody against MBP Thr232. This antibody recognizes Thr229 in mice and Thr122 in rat
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ModificationPhosphorylation
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Modification siteThr232
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ClonalityPolyclonal
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ClonePolyclonal antibody
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Concentration1ug per 1ul
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Immunogen_range225-235/304
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Subcellular locationCytoplasm
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SourceKLH conjugated synthetic phosphopeptide derived from human MBP around the phosphorylation site of Thr232
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Gene ID number4155
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Swiss ProtP02686
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Tested applicationsIF(IHC-P)
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Recommended dilutionsIF(IHC-P)(1:50-200)
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CrossreactivityHuman, Mouse, Rat
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Crossreactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen backgroundThe classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission494nm/518nm
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SynonymsMyelin basic protein; MBP; Myelin A1 protein; Myelin membrane encephalitogenic protein
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PropertiesIf you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
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Additional conjugationAnti-FITC Antibody
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French translationanticorps
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Gene target
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Gene symbolMBP, PRG2, ENO1, HIVEP2, HIVEP1
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Short nameAnti-MBP Thr232
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
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IsotypeImmunoglobulin G (IgG)
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Labelmbp
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Alternative nameAnti-MBP Thr232 PAb FITC
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Alternative techniqueantibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene namemyelin basic protein
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameproteoglycan 2, pro eosinophil major basic protein
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Synonyms gene name
- proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-09-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C-type lectin domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameenolase 1
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Synonyms gene
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Synonyms gene name
- enolase 1, (alpha)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1989-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Enolases
- MicroRNA protein coding host genes
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameHIVEP zinc finger 2
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Synonyms gene name
- human immunodeficiency virus type I enhancer-binding protein 2
- human immunodeficiency virus type I enhancer binding protein 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1992-10-21
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Entrez gene record
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Pubmed identfication
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Classification
- Zinc fingers C2H2-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameHIVEP zinc finger 1
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Synonyms gene
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Synonyms gene name
- human immunodeficiency virus type I enhancer-binding protein 1
- zinc finger protein 40
- human immunodeficiency virus type I enhancer binding protein 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1990-10-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers C2H2-type
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data